Influence of Cell Wall Polysaccharides on Structure and Mechanics of Streptococcus mutans

Joree N. Sandin, Natalia Korotkova, Martha E. Grady

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

Streptococcus mutans (S. mutans) is a group of cocci bacteria that highly contributes to oral decay. In cases of high potency, it can cause plaque build-up, impaired speech, difficulty chewing, and cavity formation, which contribute to over half of the dental visits in the United States. In severe cases where a biofilm develops on a dental implant, patients will experience pain, swelling, and potential loosening or loss of the titanium implant. S. mutans are also highly resistant to antibiotics, which makes the infections both persistent and difficult to treat. As poor oral hygiene continues to be a global epidemic, it is important to study and characterize the mechanics of the bacteria to develop therapeutic targets to alleviate S. mutans infections. One possible target is modifications of the cell wall. Recent literature suggests that alterations to the biosynthesis pathways of cell wall polysaccharides could lead to new opportunities for therapeutics. The cell wall of S. mutans is high-functioning and complex. It consists of multiple peptidoglycan layers, wall teichoic acid (WTA) containing surface glycopolymers, and a polysaccharide capsule. Using atomic force microscopy (AFM) in combination with fluorescent laser scanning confocal imaging, we compare cell wall deformation with mutants defective in WTA. Furthermore, scanning electron microscopy of both wild type S. mutans and mutant strains reveal differences in surface morphology. Our long-term goal is to determine mechanical and structural properties of bacterial cell walls that contribute to antibiotic resistance in order to preferentially regulate such properties.

Original languageEnglish
Title of host publicationMechanics of Biological Systems and Materials and Micro-and Nanomechanics, Volume 4 - Proceedings of the 2019 Annual Conference on Experimental and Applied Mechanics
EditorsMartha E. Grady
Pages73-76
Number of pages4
DOIs
StatePublished - 2020
EventSEM Annual Conference and Exposition on Experimental and Applied Mechanics, 2019 - Reno, United States
Duration: Jun 3 2019Jun 6 2019

Publication series

NameConference Proceedings of the Society for Experimental Mechanics Series
ISSN (Print)2191-5644
ISSN (Electronic)2191-5652

Conference

ConferenceSEM Annual Conference and Exposition on Experimental and Applied Mechanics, 2019
Country/TerritoryUnited States
CityReno
Period6/3/196/6/19

Bibliographical note

Funding Information:
We thank the Department of Molecular and Cellular Biochemisty for use of bacterial culture equipment and materials. AFM and confocal microscopy were carried out in the UK Light Microscopy Core user facility. The facility provides access to and expertise in a wide range of advanced imaging instrumentation that include confocal, multiphoton, and super resolution microscopes. This work was also supported by an Igniting Research Collaboration Program at the University of Kentucky.

Funding Information:
Acknowledgements We thank the Department of Molecular and Cellular Biochemisty for use of bacterial culture equipment and materials. AFM and confocal microscopy were carried out in the UK Light Microscopy Core user facility. The facility provides access to and expertise in a wide range of advanced imaging instrumentation that include confocal, multiphoton, and super resolution microscopes. This work was also supported by an Igniting Research Collaboration Program at the University of Kentucky.

Publisher Copyright:
© 2020, Society for Experimental Mechanics, Inc.

Keywords

  • Atomic force microscopy
  • Bacteria
  • Cell wall
  • Confocal microscopy
  • Streptococcus mutans

ASJC Scopus subject areas

  • Engineering (all)
  • Computational Mechanics
  • Mechanical Engineering

Fingerprint

Dive into the research topics of 'Influence of Cell Wall Polysaccharides on Structure and Mechanics of Streptococcus mutans'. Together they form a unique fingerprint.

Cite this