Influence of cigarette smoke inhalation on an autogenous onlay bone graft area in rats with estrogen deficiency: A histomorphometric and immunohistochemistry study

Camilla Magnoni Moretto Nunes, Daniella Vicensotto Bernardo, Camila Lopes Ferreira, Mônica Fernandes Gomes, Andrea Carvalho De Marco, Mauro Pedrine Santamaria, Maria Aparecida Neves Jardini

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2 Scopus citations

Abstract

Purpose: The present study aimed to evaluate the influence of cigarette smoke inhalation on an autogenous onlay bone graft area, either covered with a collagen membrane or not, in healthy and estrogen-deficient rats through histomorphometry and immunohistochemistry. Materials and Methods: Sixty female rats (Wistar), weighing 250-300 g, were randomly divided and allocated into groups (either exposed to cigarette smoke inhalation or not, ovariectomized and SHAM). After 15 days, the test group underwent cigarette smoke inhalation. Sixty days after exposition, autogenous bone grafting was only performed on all right hemimandibles, and the left ones underwent autogenous onlay bone grafting with the collagen membrane (BioGide®). The graft was harvested from the parietal bone and attached to the animals’ jaws (right and left). They were euthanized at 21, 45, and 60 days after grafting. Histological measurements and immunohistochemical analyses were performed, and results were submitted to a statistical analysis. Results: The addition of a collagen membrane to the bone graft proved more effcient in preserving graft area if compared to the graft area without a collagen membrane and the one associated with cigarette smoke inhalation at 21 (p = 0.0381) and 60 days (p = 0.0192), respectively. Cigarette smoke inhalation combined with ovariectomy promoted a significant reduction of the autogenous graft area at 21 and 60 days. At 45 days, no statistically significant results were observed. In the immunohistochemical analysis, the ovariectomized and smoking subgroups, combined or not with collagen membrane, received moderate and intense immunolabeling at 21 days for Receptor Activator of Nuclear Factor Kappa-B Ligand (RANKL) (p = 0.0017 and p = 0.0381, respectively). For Osteoprotegerin (OPG), intense immunolabeling was observed in most subgroups under analysis at 60 days. Conclusion: Smoking inhalation promoted resorption on the autogenous onlay bone graft, mainly when associated with ovariectomy. Furthermore, when associated with the collagen membrane, a lower resorption rate was observed if compared to the absence of the membrane.

Original languageEnglish
Article number1854
JournalInternational Journal of Molecular Sciences
Volume20
Issue number8
DOIs
StatePublished - Apr 2 2019

Bibliographical note

Funding Information:
Funding: This research was funded by National Council for Scientific and Technological Development from Brazil, National Institute of Science and Technology Complex Fluids (INCT-FCx)-(CNPq–465259/2014-6) and São Paulo Research Foundation (FAPESP–2014/50983-3).

Publisher Copyright:
© 2019 by the authors.

Keywords

  • Bone regeneration
  • Estrogen deficiency
  • Smoking

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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