Abstract
The ratio of milk-clotting activity to proteolytic activity (MC/PA) was used as an index to determine the quality of milk-clotting enzyme. Solid-state fermentation on wheat bran for 5 days at room temperature gave optimal production for enzyme by Rhizomucor miehei and R. pusillus. A ratio of wheat bran to moisture of 1:0.6 (w/v) gave best results. Adding skim milk powder to the media of R. miehei did not improve the MC/PA ratio but 4% (w/w) of the powder did improve the ratio with R. pusillus. Co-cultivation of R. miehei with R. pusillus did not change the MC/PA ratio.
Original language | English |
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Pages (from-to) | 527-530 |
Number of pages | 4 |
Journal | World Journal of Microbiology and Biotechnology |
Volume | 10 |
Issue number | 5 |
DOIs | |
State | Published - Sep 1994 |
Keywords
- Rhizomucor miehei
- Rhizomucor pusillus
- milk-clotting activity
- proteolytic activity
ASJC Scopus subject areas
- Biotechnology
- Physiology
- Applied Microbiology and Biotechnology