Influence of culture conditions on the production of milk-clotting enzyme from Rhizomucor

S. Preetha, R. Boopathy

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

The ratio of milk-clotting activity to proteolytic activity (MC/PA) was used as an index to determine the quality of milk-clotting enzyme. Solid-state fermentation on wheat bran for 5 days at room temperature gave optimal production for enzyme by Rhizomucor miehei and R. pusillus. A ratio of wheat bran to moisture of 1:0.6 (w/v) gave best results. Adding skim milk powder to the media of R. miehei did not improve the MC/PA ratio but 4% (w/w) of the powder did improve the ratio with R. pusillus. Co-cultivation of R. miehei with R. pusillus did not change the MC/PA ratio.

Original languageEnglish
Pages (from-to)527-530
Number of pages4
JournalWorld Journal of Microbiology and Biotechnology
Volume10
Issue number5
DOIs
StatePublished - Sep 1994

Keywords

  • Rhizomucor miehei
  • Rhizomucor pusillus
  • milk-clotting activity
  • proteolytic activity

ASJC Scopus subject areas

  • Biotechnology
  • Physiology
  • Applied Microbiology and Biotechnology

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