TY - JOUR
T1 - Influence of dietary energy intake and substrate addition on the In vitro metabolism of glucose and glutamine in Rumen epithelial tissue
AU - Harmon, D. L.
PY - 1986
Y1 - 1986
N2 - 1. 1. Ten Holstein steers were fed either 14.2 or 26.2 Mcal ME for 28 days prior to investigating the effect of dietary energy on epithelial metabolism. 2. 2. Rumen papillae were incubated in vitro with glucose (5 mM) or glutamine (1 mM) as well as additional energy substrates. 3. 3. Increased dietary intake increased production of 14CO2 from glucose and glutamine, increased uptake and net lactate production from glucose, and decreased net glutamate and alanine production from glutamine. 4. 4. At these substrate concentrations, rates of glucose oxidation to 14CO2 were sevenfold higher than glutamine.
AB - 1. 1. Ten Holstein steers were fed either 14.2 or 26.2 Mcal ME for 28 days prior to investigating the effect of dietary energy on epithelial metabolism. 2. 2. Rumen papillae were incubated in vitro with glucose (5 mM) or glutamine (1 mM) as well as additional energy substrates. 3. 3. Increased dietary intake increased production of 14CO2 from glucose and glutamine, increased uptake and net lactate production from glucose, and decreased net glutamate and alanine production from glutamine. 4. 4. At these substrate concentrations, rates of glucose oxidation to 14CO2 were sevenfold higher than glutamine.
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U2 - 10.1016/0305-0491(86)90062-3
DO - 10.1016/0305-0491(86)90062-3
M3 - Article
C2 - 3791964
AN - SCOPUS:0022986489
SN - 0305-0491
VL - 85
SP - 643
EP - 647
JO - Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology
JF - Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology
IS - 3
ER -