Influence of intra-abomasal administration of L-DOPA on circulating catecholamines and feed intake in cattle

Eriton E.L. Valente, James L. Klotz, Amanda M. Egert-McLean, Gustavo W. Costa, John B. May, David L. Harmon

Research output: Contribution to journalArticlepeer-review


Dopamine has multiple physiological functions including feed intake control in which it can act as an anorectic or orexigenic agent. This study had the objective to evaluate intra-abomasal administration of L-DOPA (levodopa; L-3,4-dihydroxyphenylalanine) from -Mucuna pruriens on circulating catecholamines, indicators of energy metabolism and feed intake in cattle. Eight Holstein steers (340 ± 20 kg) fitted with ruminal cannula were used in a replicated 4 x 4 Latin Square design experiment. Intra-abomasal infusion of L-DOPA at 0, 0.5, 1 and 2 mg/kg BW was carried out for seven days and blood samples were collected at 0, 30, 60, 120, 240 and 480 min from L-DOPA infusion on day 7. The area under the curve (AUC) of plasma L-DOPA and free dopamine increased quadratically with the administration of L-DOPA. However, the AUC of plasma total dopamine had a positive linear response with the increase of L-DOPA. Conversely, the serum 5-hydroxytriptophan (5-HTP), plasma serotonin, serum serotonin, serum tyrosine, plasma glucose and plasma free fatty acids were not affected by the intra-abomasal infusion of L-DOPA. The circulating concentration of the epinephrine, norepinephrine, serotonin, glucose and free fatty acids did not change with L-DOPA infusion. It can be concluded that intra-abomasal L-DOPA administration produced a strong increase in circulating dopamine with no change in energy metabolites and feed intake in cattle.

Original languageEnglish
Article number1127575
JournalFrontiers in Animal Science
StatePublished - 2023

Bibliographical note

Funding Information:
The authors would like to acknowledge the support of K. Vanzant, B. Cotton, and W. Lin in the conduct of this research. This work is funded by Hatch Capacity Grant Project no. KY007088 from the USDA National Institute of Food and Agriculture and Project 201807121511 from USDA/ARS and the University of Kentucky Agricultural Experiment Station.

Publisher Copyright:
Copyright © 2023 Valente, Klotz, Egert-McLean, Costa, May and Harmon.


  • dopamine
  • levodopa
  • metabolism
  • neurotransmitter
  • serotonin

ASJC Scopus subject areas

  • Animal Science and Zoology


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