Influence of proliferation on DNA repair rates in liver

William H.St Clair, B. S. Dwarakanath, Hong Zhang, Kenneth T. Wheeler

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


To test the hypothesis that the proliferative status of a mammalian cell determines the rate of removal of oxidative DNA damage, pre- and posthepatectomized livers in adult male Fisher 344 rats were irradiated in situ with 15.5 Gy of 137Cs γ-rays. At 10 and 45 min after irradiation, the livers were removed and dissociated into single cell suspensions, and the DNA damage in the isolated quiescent or proliferative liver cells was assayed by alkaline elution. Proliferative liver cells irradiated 20-24 h or 29-31 h after hepatectomy repaired their DNA damage faster than quiescent liver cells. A corresponding increase in the accessibility of the DNA to digestion by m. nuclease was observed for the posthepatectomized liver cells. These data suggest that proliferative status is a major determinant of the rate of DNA repair in rat liver.

Original languageEnglish
Pages (from-to)323-325
Number of pages3
JournalExperimental Cell Research
Issue number2
StatePublished - Dec 1991

Bibliographical note

Funding Information:
This work was supported by Grant CA-45156 from the National Cancer Institute of the National Institutes of Health. We thank Dr. T. M. Morgan of the Biostatistics Unit of the Wake Forest University Cancer Center for statistical help, Dr. C. A. Wallen for discussions, J. Anderson for technical support, and D. Cantrell for preparation of the manuscript.

ASJC Scopus subject areas

  • Cell Biology


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