Influence of prolonged serotonin and ergovaline pre-exposure on vasoconstriction ex vivo

Eriton E.L. Valente, David L. Harmon, James L. Klotz

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Ergot alkaloid mycotoxins interfere in many functions associated with serotonergic neu-rotransmitters. Therefore, the objective was to evaluate whether the association of serotonin (5-hydroxytryptamine, 5-HT) and ergot alkaloids during a 24 h pre-incubation could affect the vascular contractile response to ergot alkaloids. To evaluate the effects of 24 h exposure to 5-HT and ergot alkaloids (ergovaline, ERV), two assays were conducted. The first assay determined the half-maximal inhibitory concentration (IC50 ) following the 24 h pre-exposure period, while the second assay evalu-ated the effect of IC50 concentrations of 5-HT and ERV either individually or in combination. There was an interaction between previous exposure to 5-HT and ERV. Previous exposure to 5-HT at the IC50 concentration of 7.57 × 10−7 M reduced the contractile response by more than 50% of control, while the exposure to ERV at IC50 dose of 1.57 × 10−10 M tended to decrease (p = 0.081) vessel contractility with a response higher than 50% of control. The 24 h previous exposure to both 5-HT and ERV did not potentiate the inhibitory response of blood vessels in comparison with incubation with each compound alone. These results suggest receptor competition between 5-HT and ERV. More studies are necessary to determine the potential of 5-HT to treat toxicosis caused by ergot alkaloids.

Original languageEnglish
Article number9
JournalToxins
Volume14
Issue number1
DOIs
StatePublished - Jan 2022

Bibliographical note

Funding Information:
Funding: This work is funded by Hatch Capacity Grant Project no. KY007088 from the USDA National Institute of Food and Agriculture and Project 201807121511 from USDA/ARS and the University of Kentucky Agricultural Experiment Station.

Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

  • Blood vessel
  • Ergovaline
  • Myograph
  • Serotonin

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

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