Influence of salt and pH on the solubility and structural characteristics of transglutaminase-treated wheat gluten hydrolysate

Kingsley K. Agyare, Youling L. Xiong, Kwaku Addo

Research output: Contribution to journalArticlepeer-review

62 Scopus citations

Abstract

Hydrolyzed wheat gluten (GH, 77-85% protein) was prepared by limited chymotrypsin digestion at 37 °C for 4 h (degree of hydrolysis = 6.4%) and 15 h (degree of hydrolysis = 10.3%). Microbial transglutaminase (MTGase) treatment (55 °C for 1 h, or 5 °C for 18 h) effect on the solubility and structural characteristics of GH was examined under selected food processing conditions (pH 4.0-7.0, 0-0.6 M NaCl). The MTGase treatment increased solubility of GH by 3-29-fold (P < 0.05) within pH 4.0-7.0. Addition of 0.6 M NaCl or changing the conditions of MTGase incubation did not significantly alter solubility characteristics of GH. The MTGase treatment decreased surface hydrophobicity, and increased carboxyl groups in GH, suggesting cross-linking and deamidation. Fluorescence and UV spectra attributed the improved GH solubility to MTGase-induced polar environment, and partial masking of some nonpolar aromatic amino acids possibly due to high-molecular-weight polypeptides formed.

Original languageEnglish
Pages (from-to)1131-1137
Number of pages7
JournalFood Chemistry
Volume107
Issue number3
DOIs
StatePublished - Apr 1 2008

Keywords

  • Hydrolyzed wheat gluten
  • Microbial transglutaminase
  • Solubility
  • Structural properties

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science

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