Abstract
The mechanisms by which synaptic vesicles are transported and primed to fuse with the presynaptic membrane are important to all chemical synapses. Processes of signal transduction that affect vesicular dynamics, such as the second-messenger cascades induced by neuromodulators, are more readily addressed in assessable synaptic preparations of neuromuscular junctions in the crayfish. We assessed the effects of serotonin (5-HT) through the analysis of the latency jitter and the quantal parameters: n and p in the opener muscle of the walking leg in crayfish. There is an increase in the size of the postsynaptic currents due to more vesicles being released. Quantal analysis reveals a presynaptic mechanism by an increase in the number of vesicles being released. Latency measures show more events occur with a short latency in the presence of 5-HT. No effect on the frequency or size of spontaneous release was detected. Thus, the influence of 5-HT is presynaptic, leading to a release of more vesicles at a faster rate. Copyright (C) 2000 Elsevier Science B.V.
| Original language | English |
|---|---|
| Pages (from-to) | 16-28 |
| Number of pages | 13 |
| Journal | Brain Research |
| Volume | 871 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jul 14 2000 |
Bibliographical note
Funding Information:Funding was provided by the University of Kentucky Research and Graduate Studies Office (RLC), NSF grant IBN-9808631 (RLC), NSF-ILI-DUE 9850907 (RLC) as well as an undergraduate training fellowship from HHMI (RCS & JH) and NIH grant HL56652 (SWW).
Keywords
- Crayfish
- Neuromuscular junction
- Neurotransmission
- Serotonin
- Synapse
ASJC Scopus subject areas
- Neuroscience (all)
- Molecular Biology
- Clinical Neurology
- Developmental Biology