Influence of serotonin on the kinetics of vesicular release

R. Chase Southard, J. Haggard, M. E. Crider, S. W. Whiteheart, R. L. Cooper

Research output: Contribution to journalArticlepeer-review

36 Scopus citations


The mechanisms by which synaptic vesicles are transported and primed to fuse with the presynaptic membrane are important to all chemical synapses. Processes of signal transduction that affect vesicular dynamics, such as the second-messenger cascades induced by neuromodulators, are more readily addressed in assessable synaptic preparations of neuromuscular junctions in the crayfish. We assessed the effects of serotonin (5-HT) through the analysis of the latency jitter and the quantal parameters: n and p in the opener muscle of the walking leg in crayfish. There is an increase in the size of the postsynaptic currents due to more vesicles being released. Quantal analysis reveals a presynaptic mechanism by an increase in the number of vesicles being released. Latency measures show more events occur with a short latency in the presence of 5-HT. No effect on the frequency or size of spontaneous release was detected. Thus, the influence of 5-HT is presynaptic, leading to a release of more vesicles at a faster rate. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)16-28
Number of pages13
JournalBrain Research
Issue number1
StatePublished - Jul 14 2000

Bibliographical note

Funding Information:
Funding was provided by the University of Kentucky Research and Graduate Studies Office (RLC), NSF grant IBN-9808631 (RLC), NSF-ILI-DUE 9850907 (RLC) as well as an undergraduate training fellowship from HHMI (RCS & JH) and NIH grant HL56652 (SWW).


  • Crayfish
  • Neuromuscular junction
  • Neurotransmission
  • Serotonin
  • Synapse

ASJC Scopus subject areas

  • General Neuroscience
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology


Dive into the research topics of 'Influence of serotonin on the kinetics of vesicular release'. Together they form a unique fingerprint.

Cite this