TY - JOUR
T1 - Ingested RNA interference for managing the populations of the Colorado potato beetle, Leptinotarsa decemlineata
AU - Zhu, Fang
AU - Xu, Jingjing
AU - Palli, Roshan
AU - Ferguson, Joseph
AU - Palli, Subba R.
PY - 2011/2
Y1 - 2011/2
N2 - BACKGROUND: RNA interference (RNAi) is a breakthrough technology for conducting functional genomics studies and also as a potential tool for crop protection against insect pests. The major challenge for efficient pest control using RNAi in the field is the development of efficient and reliable methods for production and delivery of double-stranded RNA (dsRNA). In this paper, the potential of feeding dsRNA expressed in bacteria or synthesized in vitro to manage populations of Colorado potato beetle, Leptinotarsa decemlineata (Say) (CPB), was investigated.RESULTS: Feeding RNAi successfully triggered the silencing of all five target genes tested and caused significant mortality and reduced body weight gain in the treated beetles. This study provides the first example of an effective RNAi response in insects after feeding dsRNA produced in bacteria.CONCLUSION: These results suggest that the efficient induction of RNAi using bacteria to deliver dsRNA is a possible method for management of CPB. This could be also a promising bioassay approach for genome-wide screens to identify effective target genes for use as novel RNAi-based insecticides. © 2010 Society of Chemical Industry
AB - BACKGROUND: RNA interference (RNAi) is a breakthrough technology for conducting functional genomics studies and also as a potential tool for crop protection against insect pests. The major challenge for efficient pest control using RNAi in the field is the development of efficient and reliable methods for production and delivery of double-stranded RNA (dsRNA). In this paper, the potential of feeding dsRNA expressed in bacteria or synthesized in vitro to manage populations of Colorado potato beetle, Leptinotarsa decemlineata (Say) (CPB), was investigated.RESULTS: Feeding RNAi successfully triggered the silencing of all five target genes tested and caused significant mortality and reduced body weight gain in the treated beetles. This study provides the first example of an effective RNAi response in insects after feeding dsRNA produced in bacteria.CONCLUSION: These results suggest that the efficient induction of RNAi using bacteria to deliver dsRNA is a possible method for management of CPB. This could be also a promising bioassay approach for genome-wide screens to identify effective target genes for use as novel RNAi-based insecticides. © 2010 Society of Chemical Industry
KW - Bacteria-expressed dsRNA
KW - Colorado potato beetle
KW - Feeding RNAi
KW - Insecticide resistance
KW - Leptinotarsa decemlineata
KW - RNA interference
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U2 - 10.1002/ps.2048
DO - 10.1002/ps.2048
M3 - Article
C2 - 21061270
AN - SCOPUS:78650985349
SN - 1526-498X
VL - 67
SP - 175
EP - 182
JO - Pest Management Science
JF - Pest Management Science
IS - 2
ER -