Inhibition of AP-1 and Neoplastic Transformation by Fresh Apple Peel Extract

Min Ding, Yongju Lu, Linda Bowman, Chuanshu Huang, Stephen Leonard, Liying Wang, Val Vallyathan, Vince Castranova, Xianglin Shi

Research output: Contribution to journalArticlepeer-review

56 Scopus citations


Consumption of fruits and vegetables has been associated with a low incidence of cancers and other chronic diseases. Previous studies suggested that fresh apples inhibit tumor cell proliferation. Here we report that oral administration of apple peel extracts decreased the number of nonmalignant and malignant skin tumors per mouse induced by 12-O-tetradecanolyphorbol-13-acetate (TPA) in 7,12-dimethylbenz(a)anthracene-initiated mouse skin. ESR analysis indicated that apple extract strongly scavenged hydroxyl (OH) and superoxide (O2-.) radicals. Mechanistic studies showed that pretreatment with apple peel extract inhibited AP-1 transactivation induced by ultraviolet B irradiation or TPA in JB6 cells and AP-1-luciferase reporter transgenic mice. This inhibitory effect appears to be mediated by the inhibition of ERKs and JNK activity. The results provide the first evidence that an extract from fresh apple peel extract may inhibit tumor promoter-induced carcinogenesis and associated cell signaling, and suggest that the chemopreventive effects of fresh apple may be through its antioxidant properties by blocking reactive oxygen species-mediated AP-1-MAPK activation.

Original languageEnglish
Pages (from-to)10670-10676
Number of pages7
JournalJournal of Biological Chemistry
Issue number11
StatePublished - Mar 12 2004

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology


Dive into the research topics of 'Inhibition of AP-1 and Neoplastic Transformation by Fresh Apple Peel Extract'. Together they form a unique fingerprint.

Cite this