Inhibition of cigarettes smoke-induced epithelial to mesenchymal transition by the SMO inhibitor PF-5274857 in beas-2b epithelial cells

Wen Jie Zhou, Jiao Chen, Yun Feng, Ya Ping Fan, Qian Li, Jian Fu, Ping Zhang

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Objective: To explore the effects of the Smoothened (Smo) inhibitor PF-5274857 on cigarette smoke (CS)-induced epithelial-mesenchymal transition (EMT) and apply a new idea fororal squamous cell carcinoma (OSCC) treatment. Methods: Beas-2b cells were used to investigate the CS-induced EMT. Both pretreat and posttreat were performed in the study. In pretreat group, after being pretreated with 3 μmol/L PF-5274857 for 2 h, cells were cultured with CS for 8 d; In post-treat group, cells were treated by 3 μmol/L PF-5274857 for 4 d after CS cultrue. Western blot and immunofluorescence were applied to examine the EMT markers E-cadherin, vimentin, and smooth muscle actin α (α-SMA) in Beas-2b epithelial cells. The transwell culture system was used in migration assays. Results: Pretreat Beas-2b cells with PF-5274857 for 2 h can prevent the CS-induced EMT for epithelial and mesenchymal markers, as well as migration capacity. Up regulated E-cadherin and down regulated vimentin and a-SMA were observed by Western blot. Furthermore Beas-2b cells induced by CS that underwent EMT showed increased E-cadherin and decreased vimentin and rSMA after treatment with PF-5274857 for 4 d. Importantly, the elevated migration capacity level was also decreased. Conclusion: The Smo inhibitor PF-5274857 has both preventive effect and therapeutic potential against CS-induced EMT.

Original languageEnglish
Pages (from-to)485-490
Number of pages6
JournalJournal of Sichuan University (Medical Science Edition)
Volume47
Issue number4
StatePublished - Jul 2016

Keywords

  • Beas-2b epithelial cells
  • Cigarette smoke
  • Epithelial-mesenchymal transition
  • Smoothened

ASJC Scopus subject areas

  • Molecular Biology

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