TY - JOUR
T1 - Inhibition of cigarettes smoke-induced epithelial to mesenchymal transition by the SMO inhibitor PF-5274857 in beas-2b epithelial cells
AU - Zhou, Wen Jie
AU - Chen, Jiao
AU - Feng, Yun
AU - Fan, Ya Ping
AU - Li, Qian
AU - Fu, Jian
AU - Zhang, Ping
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 2016/7
Y1 - 2016/7
N2 - Objective: To explore the effects of the Smoothened (Smo) inhibitor PF-5274857 on cigarette smoke (CS)-induced epithelial-mesenchymal transition (EMT) and apply a new idea fororal squamous cell carcinoma (OSCC) treatment. Methods: Beas-2b cells were used to investigate the CS-induced EMT. Both pretreat and posttreat were performed in the study. In pretreat group, after being pretreated with 3 μmol/L PF-5274857 for 2 h, cells were cultured with CS for 8 d; In post-treat group, cells were treated by 3 μmol/L PF-5274857 for 4 d after CS cultrue. Western blot and immunofluorescence were applied to examine the EMT markers E-cadherin, vimentin, and smooth muscle actin α (α-SMA) in Beas-2b epithelial cells. The transwell culture system was used in migration assays. Results: Pretreat Beas-2b cells with PF-5274857 for 2 h can prevent the CS-induced EMT for epithelial and mesenchymal markers, as well as migration capacity. Up regulated E-cadherin and down regulated vimentin and a-SMA were observed by Western blot. Furthermore Beas-2b cells induced by CS that underwent EMT showed increased E-cadherin and decreased vimentin and rSMA after treatment with PF-5274857 for 4 d. Importantly, the elevated migration capacity level was also decreased. Conclusion: The Smo inhibitor PF-5274857 has both preventive effect and therapeutic potential against CS-induced EMT.
AB - Objective: To explore the effects of the Smoothened (Smo) inhibitor PF-5274857 on cigarette smoke (CS)-induced epithelial-mesenchymal transition (EMT) and apply a new idea fororal squamous cell carcinoma (OSCC) treatment. Methods: Beas-2b cells were used to investigate the CS-induced EMT. Both pretreat and posttreat were performed in the study. In pretreat group, after being pretreated with 3 μmol/L PF-5274857 for 2 h, cells were cultured with CS for 8 d; In post-treat group, cells were treated by 3 μmol/L PF-5274857 for 4 d after CS cultrue. Western blot and immunofluorescence were applied to examine the EMT markers E-cadherin, vimentin, and smooth muscle actin α (α-SMA) in Beas-2b epithelial cells. The transwell culture system was used in migration assays. Results: Pretreat Beas-2b cells with PF-5274857 for 2 h can prevent the CS-induced EMT for epithelial and mesenchymal markers, as well as migration capacity. Up regulated E-cadherin and down regulated vimentin and a-SMA were observed by Western blot. Furthermore Beas-2b cells induced by CS that underwent EMT showed increased E-cadherin and decreased vimentin and rSMA after treatment with PF-5274857 for 4 d. Importantly, the elevated migration capacity level was also decreased. Conclusion: The Smo inhibitor PF-5274857 has both preventive effect and therapeutic potential against CS-induced EMT.
KW - Beas-2b epithelial cells
KW - Cigarette smoke
KW - Epithelial-mesenchymal transition
KW - Smoothened
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M3 - Article
C2 - 28591947
AN - SCOPUS:84985994100
SN - 1672-173X
VL - 47
SP - 485
EP - 490
JO - Journal of Sichuan University (Medical Science Edition)
JF - Journal of Sichuan University (Medical Science Edition)
IS - 4
ER -