TY - JOUR
T1 - Inhibition of graft-versus-host disease
T2 - Use of a T cell-controlled suicide gene
AU - Lake-Bullock, M. Helene V.
AU - Bryson, J. Scott
AU - Jennings, C. Dorrell
AU - Kaplan, Alan M.
PY - 1997
Y1 - 1997
N2 - Graft-vs-host disease (GVHD) after allogeneic bone marrow transplantation is associated with significant morbidity and mortality. T cell depletion of the marrow graft, which is currently used to prevent GVHD, has been shown to result in increased graft failure and leukemia relapse. To explore the feasibility of controlling GVHD, transgenic mice with the herpes simplex virus thymidine kinase suicide gene linked to the IL-2 promoter were used as a source of T cells to induce GVHD, which would be modulated with ganciclovir. Injection of herpes simplex virus thymidine kinase transgenic B10.A(5R) spleen cells into lethally irradiated DBA/2 mice resulted in severe acute GVHD. Treatment of the recipient mice with ganciclovir significantly inhibited GVHD-mediated weight loss and mortality and reduced the severity of inflam-mation in the target organs of GVHD.
AB - Graft-vs-host disease (GVHD) after allogeneic bone marrow transplantation is associated with significant morbidity and mortality. T cell depletion of the marrow graft, which is currently used to prevent GVHD, has been shown to result in increased graft failure and leukemia relapse. To explore the feasibility of controlling GVHD, transgenic mice with the herpes simplex virus thymidine kinase suicide gene linked to the IL-2 promoter were used as a source of T cells to induce GVHD, which would be modulated with ganciclovir. Injection of herpes simplex virus thymidine kinase transgenic B10.A(5R) spleen cells into lethally irradiated DBA/2 mice resulted in severe acute GVHD. Treatment of the recipient mice with ganciclovir significantly inhibited GVHD-mediated weight loss and mortality and reduced the severity of inflam-mation in the target organs of GVHD.
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M3 - Article
C2 - 9164920
AN - SCOPUS:0031154829
SN - 0022-1767
VL - 158
SP - 5079
EP - 5082
JO - Journal of Immunology
JF - Journal of Immunology
IS - 11
ER -