In this report, we have investigated whether a protease inhibitor obtained from potatoes (chymotrypsin inhibitor 1; CI-1) will inhibit carcinogen-induced transformation of C3H/10T2-Jan cells. CI-1 was as effective as the soybean-derived Bowman Birk inhibitor at suppressing radiation-induced transformation of C3H/10T2-Jan cells, at a concentration of 10 μg/ml. The inhibitor was not toxic to the cells at concentrations of 0.1 -10 μg7sol;ml, the concentrations of CI-1 employed in the transformation experiments. To investigate the interaction of this inhibitor with the target cells, binding studies were carried out. 125I-labelled CI-1 could not be displaced from C3H7sol;10T2-Jan cells by co-incubation of the cells with a 5000-fold excess of unlabelled inhibitor. These results suggest that this inhibitor does not reversibly bind to specific receptor proteins on the surface of these cells.
|Number of pages||4|
|State||Published - Jun 1987|
Bibliographical noteFunding Information:
We thank Ms Marilyn Collins and Ms Christine Keller-McGandy for expert technical assistance. We also thank Dr Albert Owen and Dr Walter Troll for valuable suggestions concerning the work presented here. This work was supported by NTH grants CA-34680 and ES-00002.
ASJC Scopus subject areas
- Cancer Research