Inhibition of radiation-induced transformation of C3H/10T 1/2 cells by chymotrypsin inhibitor 1 from potatoes

Paul C. Billings; William St Clair; A. Ryan; Ann R. Kennedy

doi:10.1093/carcin/8.6.809

Inhibition of radiation-induced transformation of C3H/10T 1/2 cells by chymotrypsin inhibitor 1 from potatoes

Paul C. Billings
, William St Clair
, A. Ryan
, Ann R. Kennedy

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

In this report, we have investigated whether a protease inhibitor obtained from potatoes (chymotrypsin inhibitor 1; CI-1) will inhibit carcinogen-induced transformation of C3H/10T2-Jan cells. CI-1 was as effective as the soybean-derived Bowman Birk inhibitor at suppressing radiation-induced transformation of C3H/10T2-Jan cells, at a concentration of 10 μg/ml. The inhibitor was not toxic to the cells at concentrations of 0.1 -10 μg7sol;ml, the concentrations of CI-1 employed in the transformation experiments. To investigate the interaction of this inhibitor with the target cells, binding studies were carried out. ¹²⁵I-labelled CI-1 could not be displaced from C3H7sol;10T2-Jan cells by co-incubation of the cells with a 5000-fold excess of unlabelled inhibitor. These results suggest that this inhibitor does not reversibly bind to specific receptor proteins on the surface of these cells.

Original language	English
Pages (from-to)	809-812
Number of pages	4
Journal	Carcinogenesis
Volume	8
Issue number	6
DOIs	https://doi.org/10.1093/carcin/8.6.809
State	Published - Jun 1987

Bibliographical note

Funding Information:
We thank Ms Marilyn Collins and Ms Christine Keller-McGandy for expert technical assistance. We also thank Dr Albert Owen and Dr Walter Troll for valuable suggestions concerning the work presented here. This work was supported by NTH grants CA-34680 and ES-00002.

Funding

We thank Ms Marilyn Collins and Ms Christine Keller-McGandy for expert technical assistance. We also thank Dr Albert Owen and Dr Walter Troll for valuable suggestions concerning the work presented here. This work was supported by NTH grants CA-34680 and ES-00002.

Funders	Funder number
NTH	ES-00002
National Childhood Cancer Registry – National Cancer Institute	R01CA034680

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

ASJC Scopus subject areas

Cancer Research

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10.1093/carcin/8.6.809

Cite this

@article{af9e3296ac3c470cbd87bb29145776e8,

title = "Inhibition of radiation-induced transformation of C3H/10T 1/2 cells by chymotrypsin inhibitor 1 from potatoes",

abstract = "In this report, we have investigated whether a protease inhibitor obtained from potatoes (chymotrypsin inhibitor 1; CI-1) will inhibit carcinogen-induced transformation of C3H/10T2-Jan cells. CI-1 was as effective as the soybean-derived Bowman Birk inhibitor at suppressing radiation-induced transformation of C3H/10T2-Jan cells, at a concentration of 10 μg/ml. The inhibitor was not toxic to the cells at concentrations of 0.1 -10 μg7sol;ml, the concentrations of CI-1 employed in the transformation experiments. To investigate the interaction of this inhibitor with the target cells, binding studies were carried out. 125I-labelled CI-1 could not be displaced from C3H7sol;10T2-Jan cells by co-incubation of the cells with a 5000-fold excess of unlabelled inhibitor. These results suggest that this inhibitor does not reversibly bind to specific receptor proteins on the surface of these cells.",

author = "Billings, \{Paul C.\} and Clair, \{William St\} and A. Ryan and Kennedy, \{Ann R.\}",

note = "Funding Information: We thank Ms Marilyn Collins and Ms Christine Keller-McGandy for expert technical assistance. We also thank Dr Albert Owen and Dr Walter Troll for valuable suggestions concerning the work presented here. This work was supported by NTH grants CA-34680 and ES-00002.",

year = "1987",

month = jun,

doi = "10.1093/carcin/8.6.809",

language = "English",

volume = "8",

pages = "809--812",

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T1 - Inhibition of radiation-induced transformation of C3H/10T 1/2 cells by chymotrypsin inhibitor 1 from potatoes

AU - Billings, Paul C.

AU - Clair, William St

AU - Ryan, A.

AU - Kennedy, Ann R.

N1 - Funding Information: We thank Ms Marilyn Collins and Ms Christine Keller-McGandy for expert technical assistance. We also thank Dr Albert Owen and Dr Walter Troll for valuable suggestions concerning the work presented here. This work was supported by NTH grants CA-34680 and ES-00002.

PY - 1987/6

Y1 - 1987/6

N2 - In this report, we have investigated whether a protease inhibitor obtained from potatoes (chymotrypsin inhibitor 1; CI-1) will inhibit carcinogen-induced transformation of C3H/10T2-Jan cells. CI-1 was as effective as the soybean-derived Bowman Birk inhibitor at suppressing radiation-induced transformation of C3H/10T2-Jan cells, at a concentration of 10 μg/ml. The inhibitor was not toxic to the cells at concentrations of 0.1 -10 μg7sol;ml, the concentrations of CI-1 employed in the transformation experiments. To investigate the interaction of this inhibitor with the target cells, binding studies were carried out. 125I-labelled CI-1 could not be displaced from C3H7sol;10T2-Jan cells by co-incubation of the cells with a 5000-fold excess of unlabelled inhibitor. These results suggest that this inhibitor does not reversibly bind to specific receptor proteins on the surface of these cells.

AB - In this report, we have investigated whether a protease inhibitor obtained from potatoes (chymotrypsin inhibitor 1; CI-1) will inhibit carcinogen-induced transformation of C3H/10T2-Jan cells. CI-1 was as effective as the soybean-derived Bowman Birk inhibitor at suppressing radiation-induced transformation of C3H/10T2-Jan cells, at a concentration of 10 μg/ml. The inhibitor was not toxic to the cells at concentrations of 0.1 -10 μg7sol;ml, the concentrations of CI-1 employed in the transformation experiments. To investigate the interaction of this inhibitor with the target cells, binding studies were carried out. 125I-labelled CI-1 could not be displaced from C3H7sol;10T2-Jan cells by co-incubation of the cells with a 5000-fold excess of unlabelled inhibitor. These results suggest that this inhibitor does not reversibly bind to specific receptor proteins on the surface of these cells.

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AN - SCOPUS:0023197904

SN - 0143-3334

VL - 8

SP - 809

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JO - Carcinogenesis

JF - Carcinogenesis

IS - 6

ER -

Inhibition of radiation-induced transformation of C3H/10T 1/2 cells by chymotrypsin inhibitor 1 from potatoes

Abstract

Bibliographical note

Funding

UN SDGs

ASJC Scopus subject areas

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