Inhibition of [3H]dopamine uptake into rat striatal slices by quaternary n-methylated nicotine metabolites

Linda P. Dwoskin, Lora L. Leibee, Ann L. Jewell, Zhao Xia Fang, Peter A. Crooks

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


The effects of quaternary N-methylated nicotine derivatives were examined on in vitro uptake of [3H]dopamine ([3H]DA) in rat striatal slices. Striatal slices were incubated with a 10 μM concentration of the following compounds: N-methylnicotinium, N-methylnornicotinium, N-methylcotininium, N,N′ -dimethylnicotinium and N′ -methylnicotinium salts. The results clearly indicated that significant (60%) inhibition of [3H]DA uptake occurred with those compounds possessing a N-methylpyridinium group; whereas, compounds that were methylated at the N′ -pyrrolidinium position were less effective or exhibited no inhibition of [3H]DA uptake. The results suggest that high concentrations of quaternary N-methylated nicotine metabolites which are structurally related to the neurotoxin MPP+, and which may be formed in the CNS, may protect against Parkinson's Disease and explain the inverse relationship between smoking and Parkinsonism reported in epidemiologic studies.

Original languageEnglish
Pages (from-to)PL233-PL237
JournalLife Sciences
Issue number25
StatePublished - 1992

Bibliographical note

Funding Information:
This study was supported by a grant from the Tobacco and Health Institute, Lexington, KY and the R.J. Reynolds Tobacco Company.

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology (all)
  • Pharmacology, Toxicology and Pharmaceutics (all)


Dive into the research topics of 'Inhibition of [3H]dopamine uptake into rat striatal slices by quaternary n-methylated nicotine metabolites'. Together they form a unique fingerprint.

Cite this