Inhibitory activity of S-PRG filler on collagen-bound MMPs and dentin matrix degradation

Igor Paulino Mendes Soares, Caroline Anselmi, Isabela Guiné, Lídia de Oliveira Fernandes, Maria Luiza Barucci Araujo Pires, Carlos Alberto de Souza Costa, Débora Lopes Salles Scheffel, Josimeri Hebling

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Objectives: To evaluate the inhibitory activity of an ion-releasing filler (S-PRG) eluate on dentin collagen-bound metalloproteinases (MMPs) and dentin matrix degradation. Methods: Dentin beams (5 × 2 × 0.5 mm) from human molars were completely demineralized to produce dentin matrix specimens. The dry mass was measured, and a colorimetric assay (Sensolyte) determined the initial total MMP activity to allocate the beams into four treatment groups (n = 10/group): 1) water for 1 min (negative control); 2) 2% chlorhexidine digluconate (CHX – inhibitor control) for 1 min; 3) S-PRG eluate for 1 min; 4) S-PRG eluate for 30 min. After the treatments, the total MMP activity was reassessed. The specimens were stored in simulated body fluid (SBF) at 37 °C for up to 21 days. The dry mass was reassessed weekly. On day 7, the dentin matrix degradation was analyzed for the presence of collagen fragments (CF; Sirius Red) and hydroxyproline (Hyp) in the SBF. Statistical analyses were performed with ANOVA/Tukey, paired t-tests, and RM-ANOVA/Sidak (α = 5%). Results: S-PRG eluate exposure for 1 and 30 min reduced (p < 0.0001) MMP activity. S-PRG exposure for 30 min presented MMP activity inhibition equivalent to CHX (p = 0.061). S-PRG and CHX decreased CF (p ≤ 0.007) and Hyp (p < 0.046) release. After 21 days of storage, S-PRG-treated beams, regardless of exposure time, presented a reduced (p ≤ 0.017) mass loss, intermediate between CHX and control. Conclusion: Treating demineralized dentin with S-PRG eluate for 1 or 30 min reduced matrix-bound MMP activity and dentin matrix degradation for up to 21 days. Clinical significance: S-PRG filler may hinder the progression of dentin carious/erosive lesions and enhance the stabilization of dentin bonding interfaces.

Original languageEnglish
Article number104237
JournalJournal of Dentistry
StatePublished - Sep 2022

Bibliographical note

Publisher Copyright:
© 2022 Elsevier Ltd


  • Collagen
  • Dentin
  • Hydroxyproline
  • MMP
  • Pre-reacted glass filler
  • Proteolysis

ASJC Scopus subject areas

  • General Dentistry


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