TY - JOUR
T1 - Initial testing (stage 1) of the proteasome inhibitor bortezomib by the pediatric preclinical testing program
AU - Houghton, Peter J.
AU - Morton, Christopher L.
AU - Kolb, E. Anders
AU - Lock, Richard
AU - Carol, Hernan
AU - Reynolds, C. Patrick
AU - Keshelava, Nino
AU - Maris, John M.
AU - Keir, Stephen T.
AU - Wu, Jianrong
AU - Smith, Malcolm A.
PY - 2008/1
Y1 - 2008/1
N2 - Background. Bortezomib is a proteasome inhibitor that has been approved by FDA for the treatment of multiple myeloma and that has completed phase 1 testing in children. The purpose of the current study was to evaluate the antitumor activity of bortezomib against the in vitro and in vivo childhood cancer preclinical models of the Pediatric Preclinical Testing Program (PPTP). Procedures. Bortezomib was tested against the PPTP in vitro panel at concentrations ranging from 0.1 nM to 1.0 μM and was tested in vivo at a dose of 1 mg/kg for a planned duration of 6 weeks. Results. Bortezomib was uniformly active against the PPTP's in vitro panel, with a median IC50 of 23 nM and with a steep dose-response curve. The four acute lymphoblastic leukemia (ALL) cell lines had significantly lower IC50 values compared to the remaining lines of the in vitro panel. Limited in vivo activity was observed for bortezomib against the solid tumor xenografts tested, with one line meeting criteria for intermediate activity for the time to event measure and with the remaining lines showing low activity for this measure. Bortezomib demonstrated in vivo activity against the ALL panel, inducing two complete and two partial responses among seven evaluable lines. Conclusions. Administered at its MTD in mice, bortezomib demonstrated activity against selected lines of the PPTP's ALL in vivo panel. Further studies are indicated to determine the activity of bortezomib when combined with standard agents to treat childhood ALL.
AB - Background. Bortezomib is a proteasome inhibitor that has been approved by FDA for the treatment of multiple myeloma and that has completed phase 1 testing in children. The purpose of the current study was to evaluate the antitumor activity of bortezomib against the in vitro and in vivo childhood cancer preclinical models of the Pediatric Preclinical Testing Program (PPTP). Procedures. Bortezomib was tested against the PPTP in vitro panel at concentrations ranging from 0.1 nM to 1.0 μM and was tested in vivo at a dose of 1 mg/kg for a planned duration of 6 weeks. Results. Bortezomib was uniformly active against the PPTP's in vitro panel, with a median IC50 of 23 nM and with a steep dose-response curve. The four acute lymphoblastic leukemia (ALL) cell lines had significantly lower IC50 values compared to the remaining lines of the in vitro panel. Limited in vivo activity was observed for bortezomib against the solid tumor xenografts tested, with one line meeting criteria for intermediate activity for the time to event measure and with the remaining lines showing low activity for this measure. Bortezomib demonstrated in vivo activity against the ALL panel, inducing two complete and two partial responses among seven evaluable lines. Conclusions. Administered at its MTD in mice, bortezomib demonstrated activity against selected lines of the PPTP's ALL in vivo panel. Further studies are indicated to determine the activity of bortezomib when combined with standard agents to treat childhood ALL.
KW - Bortezomib
KW - Developmental therapeutics
KW - Preclinical testing
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U2 - 10.1002/pbc.21214
DO - 10.1002/pbc.21214
M3 - Article
C2 - 17420992
AN - SCOPUS:36849020943
SN - 1545-5009
VL - 50
SP - 37
EP - 45
JO - Pediatric Blood and Cancer
JF - Pediatric Blood and Cancer
IS - 1
ER -