Insect Genomics

Subba R. Palli, Hua Bai, John Wigginton

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

This chapter discusses the insect genome sequencing and analysis of sequenced genomes using "omics" and high-throughput sequencing technologies. It also provides an overview of proteomics and structural genomics of insect systems biology. Research on insects, especially in the areas of physiology, biochemistry, and molecular biology, has undergone notable transformations during the last two decades. Almost all insect genomes sequenced to date employed the whole-genome shotgun sequencing (WGS) method. Shotgun genome sequencing begins with isolation of high molecular weight genomic DNA from nuclei isolated from isogenic lines of insects. The genomic DNA is then randomly sheared, end-polished with Bal31 nuclease/ T4 DNA polymerase primers, and, finally, the DNA is size-selected. The size-selected, sheared DNA is then ligated to restriction enzyme adaptors such as the BstX1 adaptors. Subsequently, the genomic fragments are inserted into restriction enzyme-linearized plasmid vectors. Most genomes sequenced to date employed this technology. Sanger sequencing must be distinguished from next generation sequencing technology, which has entered the marketplace during the last four years and is rapidly changing the approaches used to sequence genomes. Genomes sequenced by NGS technologies will be completed more quickly and at a lower price than those from the first few insect genomes.

Original languageEnglish
Title of host publicationInsect Molecular Biology and Biochemistry
Pages1-29
Number of pages29
DOIs
StatePublished - 2012

Bibliographical note

Funding Information:
We apologize to those whose work could not be cited owing to space limitations. The research in the Palli laboratory was supported by the National Science Foundation (IBN-0421856), the National Institute of Health (GM070559-06), and the National Research Initiative of the USDA-CSREES (2007-04636). This report is contribution number 11-08-036 from the Kentucky Agricultural Experimental Station.

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (all)

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