Insights into Asparaginase from Endophytic Fungus Lasiodiplodia theobromae: Purification, Characterization and Antileukemic Activity

Hani A. Moubasher, Bassem A. Balbool, Yosra A. Helmy, Amnah Mohammed Alsuhaibani, Ahmed A. Atta, Donia H. Sheir, Ahmed M. Abdel-Azeem

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Endobiotic fungi are considered as a reservoir of numerous active metabolites. Asparaginase is used as an antileukemic drug specially to treat acute lymphoblastic leukaemia. The presented study aims to optimize the media conditions, purify, characterize, and test the antileukemic activity of the asparaginase induced from Lasiodiplodia theobromae. The culture medium was optimized using an experiment designed by The Taguchi model with an activity ranging from 10 to 175 IU/mL. Asparaginase was induced with an activity of 315 IU/mL. Asparaginase was purified with a specific activity of 468.03 U/mg and total activity of 84.4 IU/mL. The purified asparaginase showed an approximate size of 70 kDa. The purified asparaginase showed an optimum temperature of 37 °C and an optimum pH of 6. SDS reduced the activity of asparaginase to 0.65 U/mL while the used ionic surfactants enhanced the enzyme activity up to 151.92 IU/mL. The purified asparaginase showed a Km of 9.37 µM and Vmax of 127.00 µM/mL/min. The purified asparaginase showed an IC50 of 35.2 ± 0.7 IU/mL with leukemic M-NFS-60 cell lines and CC50 of 79.4 ± 1.9 IU/mL with the normal WI-38 cell line. The presented study suggests the use of endophytic fungi as a sustainable source for metabolites such as asparaginase, provides an opportunity to develop a facile, eco-friendly, costeffective, and rapid synthesis of antileukemic drugs, which have the potential to be used as alternative and reliable sources for potent anticancer agents.

Original languageEnglish
Article number680
JournalInternational Journal of Environmental Research and Public Health
Volume19
Issue number2
DOIs
StatePublished - Jan 1 2022

Bibliographical note

Funding Information:
Acknowledgments: We appreciate and thank Taif University for the financial support for Taif University Re-searchers Supporting Project (TURSP-2020/22), Taif University, Taif, Saudi Arabia. We would like to present their deep appreciation to Prof. Dr. Abdel Ghaffar Abu El Saoud, Botany and Microbiology Department, Faculty of Science, Suez Canal University for his support and help during the data analysis of the results. Also, to the members of Saint Katherine Protectorate for their support during the samples collection in the early stages of the research work progress.

Funding Information:
We appreciate and thank Taif University for the financial support for Taif University Re-searchers Supporting Project (TURSP-2020/22), Taif University, Taif, Saudi Arabia. We would like to present their deep appreciation to Prof. Dr. Abdel Ghaffar Abu El Saoud, Botany and Microbiology Department, Faculty of Science, Suez Canal University for his support and help during the data analysis of the results. Also, to the members of Saint Katherine Protectorate for their support during the samples collection in the early stages of the research work progress.

Publisher Copyright:
© 2022 by the authors. Submitted for possible open access.

Keywords

  • Antileukemia
  • Asparaginase
  • Endophytic fungi
  • Induction
  • Metabolites1
  • Purification

ASJC Scopus subject areas

  • Pollution
  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

Fingerprint

Dive into the research topics of 'Insights into Asparaginase from Endophytic Fungus Lasiodiplodia theobromae: Purification, Characterization and Antileukemic Activity'. Together they form a unique fingerprint.

Cite this