Insights into Asparaginase from Endophytic Fungus Lasiodiplodia theobromae: Purification, Characterization and Antileukemic Activity

Hani A. Moubasher; Bassem A. Balbool; Yosra A. Helmy; Amnah Mohammed Alsuhaibani; Ahmed A. Atta; Donia H. Sheir; Ahmed M. Abdel-Azeem

doi:10.3390/ijerph19020680

Insights into Asparaginase from Endophytic Fungus Lasiodiplodia theobromae: Purification, Characterization and Antileukemic Activity

Hani A. Moubasher, Bassem A. Balbool, Yosra A. Helmy, Amnah Mohammed Alsuhaibani, Ahmed A. Atta, Donia H. Sheir, Ahmed M. Abdel-Azeem

Veterinary Science

Research output: Contribution to journal › Article › peer-review

4 Citations (SciVal)

Abstract

Endobiotic fungi are considered as a reservoir of numerous active metabolites. Asparaginase is used as an antileukemic drug specially to treat acute lymphoblastic leukaemia. The presented study aims to optimize the media conditions, purify, characterize, and test the antileukemic activity of the asparaginase induced from Lasiodiplodia theobromae. The culture medium was optimized using an experiment designed by The Taguchi model with an activity ranging from 10 to 175 IU/mL. Asparaginase was induced with an activity of 315 IU/mL. Asparaginase was purified with a specific activity of 468.03 U/mg and total activity of 84.4 IU/mL. The purified asparaginase showed an approximate size of 70 kDa. The purified asparaginase showed an optimum temperature of 37 °C and an optimum pH of 6. SDS reduced the activity of asparaginase to 0.65 U/mL while the used ionic surfactants enhanced the enzyme activity up to 151.92 IU/mL. The purified asparaginase showed a Km of 9.37 µM and Vmax of 127.00 µM/mL/min. The purified asparaginase showed an IC50 of 35.2 ± 0.7 IU/mL with leukemic M-NFS-60 cell lines and CC50 of 79.4 ± 1.9 IU/mL with the normal WI-38 cell line. The presented study suggests the use of endophytic fungi as a sustainable source for metabolites such as asparaginase, provides an opportunity to develop a facile, eco-friendly, costeffective, and rapid synthesis of antileukemic drugs, which have the potential to be used as alternative and reliable sources for potent anticancer agents.

Original language	English
Article number	680
Journal	International Journal of Environmental Research and Public Health
Volume	19
Issue number	2
DOIs	https://doi.org/10.3390/ijerph19020680
State	Published - Jan 1 2022

Bibliographical note

Funding Information:
Acknowledgments: We appreciate and thank Taif University for the financial support for Taif University Re-searchers Supporting Project (TURSP-2020/22), Taif University, Taif, Saudi Arabia. We would like to present their deep appreciation to Prof. Dr. Abdel Ghaffar Abu El Saoud, Botany and Microbiology Department, Faculty of Science, Suez Canal University for his support and help during the data analysis of the results. Also, to the members of Saint Katherine Protectorate for their support during the samples collection in the early stages of the research work progress.

Funding Information:
We appreciate and thank Taif University for the financial support for Taif University Re-searchers Supporting Project (TURSP-2020/22), Taif University, Taif, Saudi Arabia. We would like to present their deep appreciation to Prof. Dr. Abdel Ghaffar Abu El Saoud, Botany and Microbiology Department, Faculty of Science, Suez Canal University for his support and help during the data analysis of the results. Also, to the members of Saint Katherine Protectorate for their support during the samples collection in the early stages of the research work progress.

Publisher Copyright:
© 2022 by the authors. Submitted for possible open access.

Keywords

Antileukemia
Asparaginase
Endophytic fungi
Induction
Metabolites1
Purification

ASJC Scopus subject areas

Pollution
Public Health, Environmental and Occupational Health
Health, Toxicology and Mutagenesis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.3390/ijerph19020680

Cite this

Moubasher, H. A., Balbool, B. A., Helmy, Y. A., Alsuhaibani, A. M., Atta, A. A., Sheir, D. H., & Abdel-Azeem, A. M. (2022). Insights into Asparaginase from Endophytic Fungus Lasiodiplodia theobromae: Purification, Characterization and Antileukemic Activity. International Journal of Environmental Research and Public Health, 19(2), [680]. https://doi.org/10.3390/ijerph19020680

@article{4fa50c41731e406a9cf65c0fd9b65037,

title = "Insights into Asparaginase from Endophytic Fungus Lasiodiplodia theobromae: Purification, Characterization and Antileukemic Activity",

abstract = "Endobiotic fungi are considered as a reservoir of numerous active metabolites. Asparaginase is used as an antileukemic drug specially to treat acute lymphoblastic leukaemia. The presented study aims to optimize the media conditions, purify, characterize, and test the antileukemic activity of the asparaginase induced from Lasiodiplodia theobromae. The culture medium was optimized using an experiment designed by The Taguchi model with an activity ranging from 10 to 175 IU/mL. Asparaginase was induced with an activity of 315 IU/mL. Asparaginase was purified with a specific activity of 468.03 U/mg and total activity of 84.4 IU/mL. The purified asparaginase showed an approximate size of 70 kDa. The purified asparaginase showed an optimum temperature of 37 °C and an optimum pH of 6. SDS reduced the activity of asparaginase to 0.65 U/mL while the used ionic surfactants enhanced the enzyme activity up to 151.92 IU/mL. The purified asparaginase showed a Km of 9.37 µM and Vmax of 127.00 µM/mL/min. The purified asparaginase showed an IC50 of 35.2 ± 0.7 IU/mL with leukemic M-NFS-60 cell lines and CC50 of 79.4 ± 1.9 IU/mL with the normal WI-38 cell line. The presented study suggests the use of endophytic fungi as a sustainable source for metabolites such as asparaginase, provides an opportunity to develop a facile, eco-friendly, costeffective, and rapid synthesis of antileukemic drugs, which have the potential to be used as alternative and reliable sources for potent anticancer agents.",

keywords = "Antileukemia, Asparaginase, Endophytic fungi, Induction, Metabolites1, Purification",

author = "Moubasher, {Hani A.} and Balbool, {Bassem A.} and Helmy, {Yosra A.} and Alsuhaibani, {Amnah Mohammed} and Atta, {Ahmed A.} and Sheir, {Donia H.} and Abdel-Azeem, {Ahmed M.}",

note = "Funding Information: Acknowledgments: We appreciate and thank Taif University for the financial support for Taif University Re-searchers Supporting Project (TURSP-2020/22), Taif University, Taif, Saudi Arabia. We would like to present their deep appreciation to Prof. Dr. Abdel Ghaffar Abu El Saoud, Botany and Microbiology Department, Faculty of Science, Suez Canal University for his support and help during the data analysis of the results. Also, to the members of Saint Katherine Protectorate for their support during the samples collection in the early stages of the research work progress. Funding Information: We appreciate and thank Taif University for the financial support for Taif University Re-searchers Supporting Project (TURSP-2020/22), Taif University, Taif, Saudi Arabia. We would like to present their deep appreciation to Prof. Dr. Abdel Ghaffar Abu El Saoud, Botany and Microbiology Department, Faculty of Science, Suez Canal University for his support and help during the data analysis of the results. Also, to the members of Saint Katherine Protectorate for their support during the samples collection in the early stages of the research work progress. Publisher Copyright: {\textcopyright} 2022 by the authors. Submitted for possible open access.",

year = "2022",

month = jan,

day = "1",

doi = "10.3390/ijerph19020680",

language = "English",

volume = "19",

number = "2",

}

Insights into Asparaginase from Endophytic Fungus Lasiodiplodia theobromae : Purification, Characterization and Antileukemic Activity. / Moubasher, Hani A.; Balbool, Bassem A.; Helmy, Yosra A. et al.

In: International Journal of Environmental Research and Public Health, Vol. 19, No. 2, 680, 01.01.2022.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Insights into Asparaginase from Endophytic Fungus Lasiodiplodia theobromae

T2 - Purification, Characterization and Antileukemic Activity

AU - Moubasher, Hani A.

AU - Balbool, Bassem A.

AU - Helmy, Yosra A.

AU - Alsuhaibani, Amnah Mohammed

AU - Atta, Ahmed A.

AU - Sheir, Donia H.

AU - Abdel-Azeem, Ahmed M.

N1 - Funding Information: Acknowledgments: We appreciate and thank Taif University for the financial support for Taif University Re-searchers Supporting Project (TURSP-2020/22), Taif University, Taif, Saudi Arabia. We would like to present their deep appreciation to Prof. Dr. Abdel Ghaffar Abu El Saoud, Botany and Microbiology Department, Faculty of Science, Suez Canal University for his support and help during the data analysis of the results. Also, to the members of Saint Katherine Protectorate for their support during the samples collection in the early stages of the research work progress. Funding Information: We appreciate and thank Taif University for the financial support for Taif University Re-searchers Supporting Project (TURSP-2020/22), Taif University, Taif, Saudi Arabia. We would like to present their deep appreciation to Prof. Dr. Abdel Ghaffar Abu El Saoud, Botany and Microbiology Department, Faculty of Science, Suez Canal University for his support and help during the data analysis of the results. Also, to the members of Saint Katherine Protectorate for their support during the samples collection in the early stages of the research work progress. Publisher Copyright: © 2022 by the authors. Submitted for possible open access.

PY - 2022/1/1

Y1 - 2022/1/1

N2 - Endobiotic fungi are considered as a reservoir of numerous active metabolites. Asparaginase is used as an antileukemic drug specially to treat acute lymphoblastic leukaemia. The presented study aims to optimize the media conditions, purify, characterize, and test the antileukemic activity of the asparaginase induced from Lasiodiplodia theobromae. The culture medium was optimized using an experiment designed by The Taguchi model with an activity ranging from 10 to 175 IU/mL. Asparaginase was induced with an activity of 315 IU/mL. Asparaginase was purified with a specific activity of 468.03 U/mg and total activity of 84.4 IU/mL. The purified asparaginase showed an approximate size of 70 kDa. The purified asparaginase showed an optimum temperature of 37 °C and an optimum pH of 6. SDS reduced the activity of asparaginase to 0.65 U/mL while the used ionic surfactants enhanced the enzyme activity up to 151.92 IU/mL. The purified asparaginase showed a Km of 9.37 µM and Vmax of 127.00 µM/mL/min. The purified asparaginase showed an IC50 of 35.2 ± 0.7 IU/mL with leukemic M-NFS-60 cell lines and CC50 of 79.4 ± 1.9 IU/mL with the normal WI-38 cell line. The presented study suggests the use of endophytic fungi as a sustainable source for metabolites such as asparaginase, provides an opportunity to develop a facile, eco-friendly, costeffective, and rapid synthesis of antileukemic drugs, which have the potential to be used as alternative and reliable sources for potent anticancer agents.

AB - Endobiotic fungi are considered as a reservoir of numerous active metabolites. Asparaginase is used as an antileukemic drug specially to treat acute lymphoblastic leukaemia. The presented study aims to optimize the media conditions, purify, characterize, and test the antileukemic activity of the asparaginase induced from Lasiodiplodia theobromae. The culture medium was optimized using an experiment designed by The Taguchi model with an activity ranging from 10 to 175 IU/mL. Asparaginase was induced with an activity of 315 IU/mL. Asparaginase was purified with a specific activity of 468.03 U/mg and total activity of 84.4 IU/mL. The purified asparaginase showed an approximate size of 70 kDa. The purified asparaginase showed an optimum temperature of 37 °C and an optimum pH of 6. SDS reduced the activity of asparaginase to 0.65 U/mL while the used ionic surfactants enhanced the enzyme activity up to 151.92 IU/mL. The purified asparaginase showed a Km of 9.37 µM and Vmax of 127.00 µM/mL/min. The purified asparaginase showed an IC50 of 35.2 ± 0.7 IU/mL with leukemic M-NFS-60 cell lines and CC50 of 79.4 ± 1.9 IU/mL with the normal WI-38 cell line. The presented study suggests the use of endophytic fungi as a sustainable source for metabolites such as asparaginase, provides an opportunity to develop a facile, eco-friendly, costeffective, and rapid synthesis of antileukemic drugs, which have the potential to be used as alternative and reliable sources for potent anticancer agents.

KW - Antileukemia

KW - Asparaginase

KW - Endophytic fungi

KW - Induction

KW - Metabolites1

KW - Purification

UR - http://www.scopus.com/inward/record.url?scp=85122251935&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85122251935&partnerID=8YFLogxK

U2 - 10.3390/ijerph19020680

DO - 10.3390/ijerph19020680

M3 - Article

C2 - 35055502

AN - SCOPUS:85122251935

VL - 19

IS - 2

M1 - 680

ER -

Insights into Asparaginase from Endophytic Fungus Lasiodiplodia theobromae: Purification, Characterization and Antileukemic Activity

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this