Intrabursal administration of protein kinase or proteinase inhibitors: Effects of ovulation in the rat

E. F. Guerre; M. R. Clark; K. N. Muse; T. E. Curry

doi:10.1016/s0015-0282(16)54430-6

Intrabursal administration of protein kinase or proteinase inhibitors: Effects of ovulation in the rat

E. F. Guerre, M. R. Clark, K. N. Muse, T. E. Curry

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Objective: To test the hypothesis that inhibition of protein kinase (PK) activity or proteolysis inhibits ovulation. Design: Rats were injected intrabursally with protein kinase (H9 or staurosporin) or proteinase (α₂-macroglobulin) inhibitors and oocyte release was evaluated. Setting: Clinical Research Laboratory, Center for Reproductive Medicine, University of Kentucky Medical Center. Participants: Immature rats stimulated with pregnant mare serum gonadotropin. Interventions: Staurosporin (1 or 10 μM), H9 (1 mM), α₂-macroglobulin (835 μIU of activity); or vehicle was injected into the right ovarian bursa. The left ovarian bursa remained intact. Animals immediately received human chorionic gonadotropin (hCG). Main Outcome Measures: Analysis of oocyte release and ovarian morphology. Results: Oocyte release from the inhibitor-treated side decreased for the H9 group (8.1 ± 1.9 fewer oocytes released, P < 0.001) and the 10 μM staurosporin group (5.5 ± 0.6, P < 0.001). No change in oocyte release was observed in the 1 μM staurosporin, α₂-macroglobulin, or vehicle injected groups. Histologic examination of vehicle treated ovaries demonstrated numerous developing corpora lutea (CL; 20.5 ± 2.1 CL/ovary) and a lack of preovulatory follicles. In contrast, ovaries treated with PK inhibitors contained unruptured preovulatory follicles coincident with fewer forming CL (11.5 ± 3.5 CL/ovary). Conclusions: Inhibition of PK activity in vivo suppresses ovulation, demonstrating that protein phosphorylation plays an important intermediary role in the ovulatory process.

Original language	English
Pages (from-to)	126-133
Number of pages	8
Journal	Fertility and Sterility
Volume	56
Issue number	1
DOIs	https://doi.org/10.1016/s0015-0282(16)54430-6
State	Published - 1991

Bibliographical note

Funding Information:
Received December 7, 1990; revised and accepted March 5, 1991. * Supported by National Institute of Health grant HD-23195, Bethesda, Maryland. t Presented at the 46th Annual Meeting of The American Fertility Society, Washington, DC, October 15 to 18,1990. t Department of Obstetrics and Gynecology, University of Kentucky. § Present address: Phoenix Center for Reproductive Medicine, Phoenix, Arizona. II Department of Obstetrics and Gynecology, University of North Carolina. ~ Department of Anatomy and Neurobiology, University of Kentucky. # Reprint requests: Thomas E. Curry, Ph.D., Department of Obstetrics and Gynecology, University of Kentucky, Lexington, Kentucky, 40536.

ASJC Scopus subject areas

Reproductive Medicine
Obstetrics and Gynecology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/s0015-0282(16)54430-6

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title = "Intrabursal administration of protein kinase or proteinase inhibitors: Effects of ovulation in the rat",

abstract = "Objective: To test the hypothesis that inhibition of protein kinase (PK) activity or proteolysis inhibits ovulation. Design: Rats were injected intrabursally with protein kinase (H9 or staurosporin) or proteinase (α2-macroglobulin) inhibitors and oocyte release was evaluated. Setting: Clinical Research Laboratory, Center for Reproductive Medicine, University of Kentucky Medical Center. Participants: Immature rats stimulated with pregnant mare serum gonadotropin. Interventions: Staurosporin (1 or 10 μM), H9 (1 mM), α2-macroglobulin (835 μIU of activity); or vehicle was injected into the right ovarian bursa. The left ovarian bursa remained intact. Animals immediately received human chorionic gonadotropin (hCG). Main Outcome Measures: Analysis of oocyte release and ovarian morphology. Results: Oocyte release from the inhibitor-treated side decreased for the H9 group (8.1 ± 1.9 fewer oocytes released, P < 0.001) and the 10 μM staurosporin group (5.5 ± 0.6, P < 0.001). No change in oocyte release was observed in the 1 μM staurosporin, α2-macroglobulin, or vehicle injected groups. Histologic examination of vehicle treated ovaries demonstrated numerous developing corpora lutea (CL; 20.5 ± 2.1 CL/ovary) and a lack of preovulatory follicles. In contrast, ovaries treated with PK inhibitors contained unruptured preovulatory follicles coincident with fewer forming CL (11.5 ± 3.5 CL/ovary). Conclusions: Inhibition of PK activity in vivo suppresses ovulation, demonstrating that protein phosphorylation plays an important intermediary role in the ovulatory process.",

author = "Guerre, {E. F.} and Clark, {M. R.} and Muse, {K. N.} and Curry, {T. E.}",

note = "Funding Information: Received December 7, 1990; revised and accepted March 5, 1991. * Supported by National Institute of Health grant HD-23195, Bethesda, Maryland. t Presented at the 46th Annual Meeting of The American Fertility Society, Washington, DC, October 15 to 18,1990. t Department of Obstetrics and Gynecology, University of Kentucky. § Present address: Phoenix Center for Reproductive Medicine, Phoenix, Arizona. II Department of Obstetrics and Gynecology, University of North Carolina. ~ Department of Anatomy and Neurobiology, University of Kentucky. # Reprint requests: Thomas E. Curry, Ph.D., Department of Obstetrics and Gynecology, University of Kentucky, Lexington, Kentucky, 40536.",

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T2 - Effects of ovulation in the rat

AU - Guerre, E. F.

AU - Clark, M. R.

AU - Muse, K. N.

AU - Curry, T. E.

N1 - Funding Information: Received December 7, 1990; revised and accepted March 5, 1991. * Supported by National Institute of Health grant HD-23195, Bethesda, Maryland. t Presented at the 46th Annual Meeting of The American Fertility Society, Washington, DC, October 15 to 18,1990. t Department of Obstetrics and Gynecology, University of Kentucky. § Present address: Phoenix Center for Reproductive Medicine, Phoenix, Arizona. II Department of Obstetrics and Gynecology, University of North Carolina. ~ Department of Anatomy and Neurobiology, University of Kentucky. # Reprint requests: Thomas E. Curry, Ph.D., Department of Obstetrics and Gynecology, University of Kentucky, Lexington, Kentucky, 40536.

PY - 1991

Y1 - 1991

N2 - Objective: To test the hypothesis that inhibition of protein kinase (PK) activity or proteolysis inhibits ovulation. Design: Rats were injected intrabursally with protein kinase (H9 or staurosporin) or proteinase (α2-macroglobulin) inhibitors and oocyte release was evaluated. Setting: Clinical Research Laboratory, Center for Reproductive Medicine, University of Kentucky Medical Center. Participants: Immature rats stimulated with pregnant mare serum gonadotropin. Interventions: Staurosporin (1 or 10 μM), H9 (1 mM), α2-macroglobulin (835 μIU of activity); or vehicle was injected into the right ovarian bursa. The left ovarian bursa remained intact. Animals immediately received human chorionic gonadotropin (hCG). Main Outcome Measures: Analysis of oocyte release and ovarian morphology. Results: Oocyte release from the inhibitor-treated side decreased for the H9 group (8.1 ± 1.9 fewer oocytes released, P < 0.001) and the 10 μM staurosporin group (5.5 ± 0.6, P < 0.001). No change in oocyte release was observed in the 1 μM staurosporin, α2-macroglobulin, or vehicle injected groups. Histologic examination of vehicle treated ovaries demonstrated numerous developing corpora lutea (CL; 20.5 ± 2.1 CL/ovary) and a lack of preovulatory follicles. In contrast, ovaries treated with PK inhibitors contained unruptured preovulatory follicles coincident with fewer forming CL (11.5 ± 3.5 CL/ovary). Conclusions: Inhibition of PK activity in vivo suppresses ovulation, demonstrating that protein phosphorylation plays an important intermediary role in the ovulatory process.

AB - Objective: To test the hypothesis that inhibition of protein kinase (PK) activity or proteolysis inhibits ovulation. Design: Rats were injected intrabursally with protein kinase (H9 or staurosporin) or proteinase (α2-macroglobulin) inhibitors and oocyte release was evaluated. Setting: Clinical Research Laboratory, Center for Reproductive Medicine, University of Kentucky Medical Center. Participants: Immature rats stimulated with pregnant mare serum gonadotropin. Interventions: Staurosporin (1 or 10 μM), H9 (1 mM), α2-macroglobulin (835 μIU of activity); or vehicle was injected into the right ovarian bursa. The left ovarian bursa remained intact. Animals immediately received human chorionic gonadotropin (hCG). Main Outcome Measures: Analysis of oocyte release and ovarian morphology. Results: Oocyte release from the inhibitor-treated side decreased for the H9 group (8.1 ± 1.9 fewer oocytes released, P < 0.001) and the 10 μM staurosporin group (5.5 ± 0.6, P < 0.001). No change in oocyte release was observed in the 1 μM staurosporin, α2-macroglobulin, or vehicle injected groups. Histologic examination of vehicle treated ovaries demonstrated numerous developing corpora lutea (CL; 20.5 ± 2.1 CL/ovary) and a lack of preovulatory follicles. In contrast, ovaries treated with PK inhibitors contained unruptured preovulatory follicles coincident with fewer forming CL (11.5 ± 3.5 CL/ovary). Conclusions: Inhibition of PK activity in vivo suppresses ovulation, demonstrating that protein phosphorylation plays an important intermediary role in the ovulatory process.

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Intrabursal administration of protein kinase or proteinase inhibitors: Effects of ovulation in the rat

Abstract

Bibliographical note

ASJC Scopus subject areas

UN SDGs

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