Intrabursal administration of protein kinase or proteinase inhibitors: Effects of ovulation in the rat

E. F. Guerre, M. R. Clark, K. N. Muse, T. E. Curry

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Objective: To test the hypothesis that inhibition of protein kinase (PK) activity or proteolysis inhibits ovulation. Design: Rats were injected intrabursally with protein kinase (H9 or staurosporin) or proteinase (α2-macroglobulin) inhibitors and oocyte release was evaluated. Setting: Clinical Research Laboratory, Center for Reproductive Medicine, University of Kentucky Medical Center. Participants: Immature rats stimulated with pregnant mare serum gonadotropin. Interventions: Staurosporin (1 or 10 μM), H9 (1 mM), α2-macroglobulin (835 μIU of activity); or vehicle was injected into the right ovarian bursa. The left ovarian bursa remained intact. Animals immediately received human chorionic gonadotropin (hCG). Main Outcome Measures: Analysis of oocyte release and ovarian morphology. Results: Oocyte release from the inhibitor-treated side decreased for the H9 group (8.1 ± 1.9 fewer oocytes released, P < 0.001) and the 10 μM staurosporin group (5.5 ± 0.6, P < 0.001). No change in oocyte release was observed in the 1 μM staurosporin, α2-macroglobulin, or vehicle injected groups. Histologic examination of vehicle treated ovaries demonstrated numerous developing corpora lutea (CL; 20.5 ± 2.1 CL/ovary) and a lack of preovulatory follicles. In contrast, ovaries treated with PK inhibitors contained unruptured preovulatory follicles coincident with fewer forming CL (11.5 ± 3.5 CL/ovary). Conclusions: Inhibition of PK activity in vivo suppresses ovulation, demonstrating that protein phosphorylation plays an important intermediary role in the ovulatory process.

Original languageEnglish
Pages (from-to)126-133
Number of pages8
JournalFertility and Sterility
Volume56
Issue number1
DOIs
StatePublished - 1991

Bibliographical note

Funding Information:
Received December 7, 1990; revised and accepted March 5, 1991. * Supported by National Institute of Health grant HD-23195, Bethesda, Maryland. t Presented at the 46th Annual Meeting of The American Fertility Society, Washington, DC, October 15 to 18,1990. t Department of Obstetrics and Gynecology, University of Kentucky. § Present address: Phoenix Center for Reproductive Medicine, Phoenix, Arizona. II Department of Obstetrics and Gynecology, University of North Carolina. ~ Department of Anatomy and Neurobiology, University of Kentucky. # Reprint requests: Thomas E. Curry, Ph.D., Department of Obstetrics and Gynecology, University of Kentucky, Lexington, Kentucky, 40536.

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

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