Intracellular localization of SNAP-23 to endosomal compartments

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22 Scopus citations


We have reexamined the intracellular localization of the ubiquitously expressed target membrane SNAP receptor (t-SNARE), SNAP-23. While SNAP-23 appears on the plasma membrane, in the cell types examined there is a significant pool associated with endosomal compartments. Immune-staining and expression of green fluorescent protein-tagged SNAP-23, show that it has a punctate, perinuclear localization in HepG2 and HT4 cells. This distribution overlaps significantly with transferrin receptor and slightly with the late endosome/lysosomal protein LAMP-1. The localization of SNAP-23 changes as HepG2 cells polarize. Initially it is concentrated at sites of cell-cell contact and then almost exclusively to the apical (or bile canalicular) domain of the cell. These data are consistent with a role for SNAP-23 in both endosome-plasma membrane trafficking as well as endosome-endosome transport.

Original languageEnglish
Pages (from-to)340-346
Number of pages7
JournalBiochemical and Biophysical Research Communications
Issue number2
StatePublished - Feb 16 1999

Bibliographical note

Funding Information:
We thank the members of the Whiteheart Laboratory: Audrey Bernstein, Paula Lemons, Elena Matveeva, Ping He, Tara Rutledge, Kris Jones, Todd Schraw, and Susan Buhrow for their helpful comments on this manuscript. This work is supported by a grant from the National Institutes of Health to S.W.W.


  • Endocytosis
  • Endosome
  • Membrane trafficking
  • SNAP-23
  • t-SNAREs

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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