Abstract
We have reexamined the intracellular localization of the ubiquitously expressed target membrane SNAP receptor (t-SNARE), SNAP-23. While SNAP-23 appears on the plasma membrane, in the cell types examined there is a significant pool associated with endosomal compartments. Immune-staining and expression of green fluorescent protein-tagged SNAP-23, show that it has a punctate, perinuclear localization in HepG2 and HT4 cells. This distribution overlaps significantly with transferrin receptor and slightly with the late endosome/lysosomal protein LAMP-1. The localization of SNAP-23 changes as HepG2 cells polarize. Initially it is concentrated at sites of cell-cell contact and then almost exclusively to the apical (or bile canalicular) domain of the cell. These data are consistent with a role for SNAP-23 in both endosome-plasma membrane trafficking as well as endosome-endosome transport.
| Original language | English |
|---|---|
| Pages (from-to) | 340-346 |
| Number of pages | 7 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 255 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 16 1999 |
Bibliographical note
Funding Information:We thank the members of the Whiteheart Laboratory: Audrey Bernstein, Paula Lemons, Elena Matveeva, Ping He, Tara Rutledge, Kris Jones, Todd Schraw, and Susan Buhrow for their helpful comments on this manuscript. This work is supported by a grant from the National Institutes of Health to S.W.W.
Keywords
- Endocytosis
- Endosome
- Membrane trafficking
- SNAP-23
- t-SNAREs
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology