Intracellular localization of SNAP-23 to endosomal compartments

Dong Chen; Sidney W. Whiteheart

doi:10.1006/bbrc.1999.0173

Intracellular localization of SNAP-23 to endosomal compartments

Dong Chen
, Sidney W. Whiteheart

Molecular and Cellular Biochemistry

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

We have reexamined the intracellular localization of the ubiquitously expressed target membrane SNAP receptor (t-SNARE), SNAP-23. While SNAP-23 appears on the plasma membrane, in the cell types examined there is a significant pool associated with endosomal compartments. Immune-staining and expression of green fluorescent protein-tagged SNAP-23, show that it has a punctate, perinuclear localization in HepG2 and HT4 cells. This distribution overlaps significantly with transferrin receptor and slightly with the late endosome/lysosomal protein LAMP-1. The localization of SNAP-23 changes as HepG2 cells polarize. Initially it is concentrated at sites of cell-cell contact and then almost exclusively to the apical (or bile canalicular) domain of the cell. These data are consistent with a role for SNAP-23 in both endosome-plasma membrane trafficking as well as endosome-endosome transport.

Original language	English
Pages (from-to)	340-346
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	255
Issue number	2
DOIs	https://doi.org/10.1006/bbrc.1999.0173
State	Published - Feb 16 1999

Bibliographical note

Funding Information:
We thank the members of the Whiteheart Laboratory: Audrey Bernstein, Paula Lemons, Elena Matveeva, Ping He, Tara Rutledge, Kris Jones, Todd Schraw, and Susan Buhrow for their helpful comments on this manuscript. This work is supported by a grant from the National Institutes of Health to S.W.W.

Funding

We thank the members of the Whiteheart Laboratory: Audrey Bernstein, Paula Lemons, Elena Matveeva, Ping He, Tara Rutledge, Kris Jones, Todd Schraw, and Susan Buhrow for their helpful comments on this manuscript. This work is supported by a grant from the National Institutes of Health to S.W.W.

Funders
National Institutes of Health (NIH)

Keywords

Endocytosis
Endosome
Membrane trafficking
SNAP-23
t-SNAREs

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1006/bbrc.1999.0173

Cite this

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title = "Intracellular localization of SNAP-23 to endosomal compartments",

abstract = "We have reexamined the intracellular localization of the ubiquitously expressed target membrane SNAP receptor (t-SNARE), SNAP-23. While SNAP-23 appears on the plasma membrane, in the cell types examined there is a significant pool associated with endosomal compartments. Immune-staining and expression of green fluorescent protein-tagged SNAP-23, show that it has a punctate, perinuclear localization in HepG2 and HT4 cells. This distribution overlaps significantly with transferrin receptor and slightly with the late endosome/lysosomal protein LAMP-1. The localization of SNAP-23 changes as HepG2 cells polarize. Initially it is concentrated at sites of cell-cell contact and then almost exclusively to the apical (or bile canalicular) domain of the cell. These data are consistent with a role for SNAP-23 in both endosome-plasma membrane trafficking as well as endosome-endosome transport.",

keywords = "Endocytosis, Endosome, Membrane trafficking, SNAP-23, t-SNAREs",

author = "Dong Chen and Whiteheart, \{Sidney W.\}",

note = "Funding Information: We thank the members of the Whiteheart Laboratory: Audrey Bernstein, Paula Lemons, Elena Matveeva, Ping He, Tara Rutledge, Kris Jones, Todd Schraw, and Susan Buhrow for their helpful comments on this manuscript. This work is supported by a grant from the National Institutes of Health to S.W.W.",

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AU - Chen, Dong

AU - Whiteheart, Sidney W.

N1 - Funding Information: We thank the members of the Whiteheart Laboratory: Audrey Bernstein, Paula Lemons, Elena Matveeva, Ping He, Tara Rutledge, Kris Jones, Todd Schraw, and Susan Buhrow for their helpful comments on this manuscript. This work is supported by a grant from the National Institutes of Health to S.W.W.

PY - 1999/2/16

Y1 - 1999/2/16

N2 - We have reexamined the intracellular localization of the ubiquitously expressed target membrane SNAP receptor (t-SNARE), SNAP-23. While SNAP-23 appears on the plasma membrane, in the cell types examined there is a significant pool associated with endosomal compartments. Immune-staining and expression of green fluorescent protein-tagged SNAP-23, show that it has a punctate, perinuclear localization in HepG2 and HT4 cells. This distribution overlaps significantly with transferrin receptor and slightly with the late endosome/lysosomal protein LAMP-1. The localization of SNAP-23 changes as HepG2 cells polarize. Initially it is concentrated at sites of cell-cell contact and then almost exclusively to the apical (or bile canalicular) domain of the cell. These data are consistent with a role for SNAP-23 in both endosome-plasma membrane trafficking as well as endosome-endosome transport.

AB - We have reexamined the intracellular localization of the ubiquitously expressed target membrane SNAP receptor (t-SNARE), SNAP-23. While SNAP-23 appears on the plasma membrane, in the cell types examined there is a significant pool associated with endosomal compartments. Immune-staining and expression of green fluorescent protein-tagged SNAP-23, show that it has a punctate, perinuclear localization in HepG2 and HT4 cells. This distribution overlaps significantly with transferrin receptor and slightly with the late endosome/lysosomal protein LAMP-1. The localization of SNAP-23 changes as HepG2 cells polarize. Initially it is concentrated at sites of cell-cell contact and then almost exclusively to the apical (or bile canalicular) domain of the cell. These data are consistent with a role for SNAP-23 in both endosome-plasma membrane trafficking as well as endosome-endosome transport.

KW - Endocytosis

KW - Endosome

KW - Membrane trafficking

KW - SNAP-23

KW - t-SNAREs

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VL - 255

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JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

Intracellular localization of SNAP-23 to endosomal compartments

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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