Intrinsic differences in brain and spinal cord mitochondria: Implication for therapeutic interventions

Patrick G. Sullivan, Alexander G. Rabchevsky, Jeffery N. Keller, Mark Lovell, Ajeet Sodhi, Ronald P. Hart, Stephen W. Scheff

Research output: Contribution to journalArticlepeer-review

102 Scopus citations


It is well known that regions of the CNS differentially respond to insults. After brain injury, cyclosporine A reduces damage but is ineffective following spinal cord injury. We address this disparity by assessing several parameters of mitochondrial physiology in the normal neocortex and spinal cord. In situ measurements of O2-. production, lipid peroxidation, and mitochondrial DNA oxidation revealed significantly higher levels in spinal cord vs. neocortical neurons. Real-time PCR demonstrated differences in mitochondrial transcripts coupled with decreases in complex I enzyme activity and respiration in spinal cord mitochondria. The threshold for calcium-induced mitochondrial permeability transition was substantially reduced in spinal cord vs. neocortex and modulated by lipid peroxidation. These intrinsic differences may provide a pivotal target for strategies to ameliorate neuronal damage following injury, and this imbalance in oxidative stress may contribute to the susceptibility of spinal cord motor neurons in neuropathologies such as amyotrophic lateral sclerosis. 2004 Wiley-Liss, Inc.

Original languageEnglish
Pages (from-to)524-534
Number of pages11
JournalJournal of Comparative Neurology
Issue number4
StatePublished - Jun 2004


  • CNS
  • Mitochondrial physiology
  • Motor neurons
  • Reactive oxygen species

ASJC Scopus subject areas

  • Neuroscience (all)


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