Investigating sesquiterpene biosynthesis in Ginkgo biloba: molecular cloning and functional characterization of (E,E)-farnesol and α-bisabolene synthases

Iffat Parveen, Mei Wang, Jianping Zhao, Amar G. Chittiboyina, Nurhayat Tabanca, Abbas Ali, Scott R. Baerson, Natascha Techen, Joe Chappell, Ikhlas A. Khan, Zhiqiang Pan

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Ginkgo biloba is one of the oldest living tree species and has been extensively investigated as a source of bioactive natural compounds, including bioactive flavonoids, diterpene lactones, terpenoids and polysaccharides which accumulate in foliar tissues. Despite this chemical diversity, relatively few enzymes associated with any biosynthetic pathway from ginkgo have been characterized to date. In the present work, predicted transcripts potentially encoding enzymes associated with the biosynthesis of diterpenoid and terpenoid compounds, including putative terpene synthases, were first identified by mining publicly-available G. biloba RNA-seq data sets. Recombinant enzyme studies with two of the TPS-like sequences led to the identification of GbTPS1 and GbTPS2, encoding farnesol and bisabolene synthases, respectively. Additionally, the phylogenetic analysis revealed the two terpene synthase genes as primitive genes that might have evolved from an ancestral diterpene synthase.

Original languageEnglish
Pages (from-to)451-462
Number of pages12
JournalPlant Molecular Biology
Volume89
Issue number4-5
DOIs
StatePublished - Oct 6 2015

Keywords

  • Bisabolene synthase
  • Farnesol synthase
  • Ginkgo biloba
  • Sesquiterpene

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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