Investigation of enzyme formulation on pretreated switchgrass

Matthew Falls; Jian Shi; Mirvat A. Ebrik; Tim Redmond; Bin Yang; Charles E. Wyman; Rebecca Garlock; Venkatesh Balan; Bruce E. Dale; V. Ramesh Pallapolu; Y. Y. Lee; Youngmi Kim; Nathan S. Mosier; Michael R. Ladisch; Bonnie Hames; Steve Thomas; Bryon S. Donohoe; Todd B. Vinzant; Richard T. Elander; Ryan E. Warner; Rocio Sierra-Ramirez; Mark T. Holtzapple

doi:10.1016/j.biortech.2011.03.035

Investigation of enzyme formulation on pretreated switchgrass

Matthew Falls, Jian Shi, Mirvat A. Ebrik, Tim Redmond, Bin Yang, Charles E. Wyman, Rebecca Garlock, Venkatesh Balan, Bruce E. Dale, V. Ramesh Pallapolu, Y. Y. Lee, Youngmi Kim, Nathan S. Mosier, Michael R. Ladisch, Bonnie Hames, Steve Thomas, Bryon S. Donohoe, Todd B. Vinzant, Richard T. Elander, Ryan E. WarnerRocio Sierra-Ramirez, Mark T. Holtzapple

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

This work studied the benefits of adding different enzyme cocktails (cellulase, xylanase, β-glucosidase) to pretreated switchgrass. Pretreatment methods included ammonia fiber expansion (AFEX), dilute-acid (DA), liquid hot water (LHW), lime, lime+ball-milling, soaking in aqueous ammonia (SAA), and sulfur dioxide (SO ₂). The compositions of the pretreated materials were analyzed and showed a strong correlation between initial xylan composition and the benefits of xylanase addition. Adding xylanase dramatically improved xylan yields for SAA (+8.4%) and AFEX (+6.3%), and showed negligible improvement (0-2%) for the pretreatments with low xylan content (dilute-acid, SO ₂). Xylanase addition also improved overall yields with lime+ball-milling and SO ₂ achieving the highest overall yields from pretreated biomass (98.3% and 93.2%, respectively). Lime+ball-milling obtained an enzymatic yield of 92.3kg of sugar digested/kg of protein loaded.

Original language	English
Pages (from-to)	11072-11079
Number of pages	8
Journal	Bioresource Technology
Volume	102
Issue number	24
DOIs	https://doi.org/10.1016/j.biortech.2011.03.035
State	Published - Dec 2011

Bibliographical note

Funding Information:
This work was supported by the US Department of Energy, contract number DE-FG36-07GO17102.

Funding

This work was supported by the US Department of Energy, contract number DE-FG36-07GO17102.

Funders	Funder number
US Department of Energy	DE-FG36-07GO17102

Keywords

Enzymatic digestion
Pretreatment
Switchgrass
Xylanase
β-glucosidase

ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.biortech.2011.03.035

Cite this

Falls, M., Shi, J., Ebrik, M. A., Redmond, T., Yang, B., Wyman, C. E., Garlock, R., Balan, V., Dale, B. E., Pallapolu, V. R., Lee, Y. Y., Kim, Y., Mosier, N. S., Ladisch, M. R., Hames, B., Thomas, S., Donohoe, B. S., Vinzant, T. B., Elander, R. T., ... Holtzapple, M. T. (2011). Investigation of enzyme formulation on pretreated switchgrass. Bioresource Technology, 102(24), 11072-11079. https://doi.org/10.1016/j.biortech.2011.03.035

@article{9b333341e5b541d491ca4b1b53baef4e,

title = "Investigation of enzyme formulation on pretreated switchgrass",

abstract = "This work studied the benefits of adding different enzyme cocktails (cellulase, xylanase, β-glucosidase) to pretreated switchgrass. Pretreatment methods included ammonia fiber expansion (AFEX), dilute-acid (DA), liquid hot water (LHW), lime, lime+ball-milling, soaking in aqueous ammonia (SAA), and sulfur dioxide (SO 2). The compositions of the pretreated materials were analyzed and showed a strong correlation between initial xylan composition and the benefits of xylanase addition. Adding xylanase dramatically improved xylan yields for SAA (+8.4%) and AFEX (+6.3%), and showed negligible improvement (0-2%) for the pretreatments with low xylan content (dilute-acid, SO 2). Xylanase addition also improved overall yields with lime+ball-milling and SO 2 achieving the highest overall yields from pretreated biomass (98.3% and 93.2%, respectively). Lime+ball-milling obtained an enzymatic yield of 92.3kg of sugar digested/kg of protein loaded.",

keywords = "Enzymatic digestion, Pretreatment, Switchgrass, Xylanase, β-glucosidase",

author = "Matthew Falls and Jian Shi and Ebrik, {Mirvat A.} and Tim Redmond and Bin Yang and Wyman, {Charles E.} and Rebecca Garlock and Venkatesh Balan and Dale, {Bruce E.} and Pallapolu, {V. Ramesh} and Lee, {Y. Y.} and Youngmi Kim and Mosier, {Nathan S.} and Ladisch, {Michael R.} and Bonnie Hames and Steve Thomas and Donohoe, {Bryon S.} and Vinzant, {Todd B.} and Elander, {Richard T.} and Warner, {Ryan E.} and Rocio Sierra-Ramirez and Holtzapple, {Mark T.}",

note = "Funding Information: This work was supported by the US Department of Energy, contract number DE-FG36-07GO17102. ",

year = "2011",

month = dec,

doi = "10.1016/j.biortech.2011.03.035",

language = "English",

volume = "102",

pages = "11072--11079",

number = "24",

}

Falls, M, Shi, J, Ebrik, MA, Redmond, T, Yang, B, Wyman, CE, Garlock, R, Balan, V, Dale, BE, Pallapolu, VR, Lee, YY, Kim, Y, Mosier, NS, Ladisch, MR, Hames, B, Thomas, S, Donohoe, BS, Vinzant, TB, Elander, RT, Warner, RE, Sierra-Ramirez, R & Holtzapple, MT 2011, 'Investigation of enzyme formulation on pretreated switchgrass', Bioresource Technology, vol. 102, no. 24, pp. 11072-11079. https://doi.org/10.1016/j.biortech.2011.03.035

TY - JOUR

T1 - Investigation of enzyme formulation on pretreated switchgrass

AU - Falls, Matthew

AU - Shi, Jian

AU - Ebrik, Mirvat A.

AU - Redmond, Tim

AU - Yang, Bin

AU - Wyman, Charles E.

AU - Garlock, Rebecca

AU - Balan, Venkatesh

AU - Dale, Bruce E.

AU - Pallapolu, V. Ramesh

AU - Lee, Y. Y.

AU - Kim, Youngmi

AU - Mosier, Nathan S.

AU - Ladisch, Michael R.

AU - Hames, Bonnie

AU - Thomas, Steve

AU - Donohoe, Bryon S.

AU - Vinzant, Todd B.

AU - Elander, Richard T.

AU - Warner, Ryan E.

AU - Sierra-Ramirez, Rocio

AU - Holtzapple, Mark T.

N1 - Funding Information: This work was supported by the US Department of Energy, contract number DE-FG36-07GO17102.

PY - 2011/12

Y1 - 2011/12

N2 - This work studied the benefits of adding different enzyme cocktails (cellulase, xylanase, β-glucosidase) to pretreated switchgrass. Pretreatment methods included ammonia fiber expansion (AFEX), dilute-acid (DA), liquid hot water (LHW), lime, lime+ball-milling, soaking in aqueous ammonia (SAA), and sulfur dioxide (SO 2). The compositions of the pretreated materials were analyzed and showed a strong correlation between initial xylan composition and the benefits of xylanase addition. Adding xylanase dramatically improved xylan yields for SAA (+8.4%) and AFEX (+6.3%), and showed negligible improvement (0-2%) for the pretreatments with low xylan content (dilute-acid, SO 2). Xylanase addition also improved overall yields with lime+ball-milling and SO 2 achieving the highest overall yields from pretreated biomass (98.3% and 93.2%, respectively). Lime+ball-milling obtained an enzymatic yield of 92.3kg of sugar digested/kg of protein loaded.

AB - This work studied the benefits of adding different enzyme cocktails (cellulase, xylanase, β-glucosidase) to pretreated switchgrass. Pretreatment methods included ammonia fiber expansion (AFEX), dilute-acid (DA), liquid hot water (LHW), lime, lime+ball-milling, soaking in aqueous ammonia (SAA), and sulfur dioxide (SO 2). The compositions of the pretreated materials were analyzed and showed a strong correlation between initial xylan composition and the benefits of xylanase addition. Adding xylanase dramatically improved xylan yields for SAA (+8.4%) and AFEX (+6.3%), and showed negligible improvement (0-2%) for the pretreatments with low xylan content (dilute-acid, SO 2). Xylanase addition also improved overall yields with lime+ball-milling and SO 2 achieving the highest overall yields from pretreated biomass (98.3% and 93.2%, respectively). Lime+ball-milling obtained an enzymatic yield of 92.3kg of sugar digested/kg of protein loaded.

KW - Enzymatic digestion

KW - Pretreatment

KW - Switchgrass

KW - Xylanase

KW - β-glucosidase

UR - http://www.scopus.com/inward/record.url?scp=80052735555&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80052735555&partnerID=8YFLogxK

U2 - 10.1016/j.biortech.2011.03.035

DO - 10.1016/j.biortech.2011.03.035

M3 - Article

C2 - 21478012

AN - SCOPUS:80052735555

SN - 0960-8524

VL - 102

SP - 11072

EP - 11079

JO - Bioresource Technology

JF - Bioresource Technology

IS - 24

ER -

Investigation of enzyme formulation on pretreated switchgrass

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this