Investigation of peroxynitrite induced oxidative stress in red blood cells monitored by luminol-dependent chemiluminescence

Jaroslaw Kanski, Tanuja Koppal, D. Allan Butterfield

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Peroxynitrite-induced oxidative stress was investigated in red blood cells by luminol-dependent chemiluminescence. Red blood cells are susceptible to free radical damage due to the abundance of oxygen, presence of iron and other agents such as polyunsaturated fatty acids. Peroxynitrite (ONOO-), formed by the reaction of superoxide radical with nitric oxide radical, has been shown to cause protein and lipid oxidation. Addition of ONOO- showed a dramatic increase in chemiluminescence, but treatment with glutathione ethyl ester, an antioxidant, partially reduced the increase in chemiluminescence. The results show that red blood cells are a good model to mimic oxidative stress conditions and are discussed with relevance to neurodegenerate disorders.

Original languageEnglish
Pages (from-to)1183-1192
Number of pages10
JournalAnalytical Letters
Volume32
Issue number6
DOIs
StatePublished - 1999

Bibliographical note

Funding Information:
ACKNOWLEDGEMENTS This work was supported in part by NIH grants (AG-10836; AG-05119). The authors would like to express sincere gratitude to the persons who volunteered to donate their blood for the experiment and to Dr. Sylvia Daunert for use of the luminometer.

Keywords

  • Chemiluminescence
  • Glutathione (GSH)
  • Luminol
  • Oxidative Stress (ROS)
  • Peroxynitrite
  • Red Blood Cells

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Spectroscopy
  • Clinical Biochemistry
  • Biochemistry, medical
  • Electrochemistry

Fingerprint

Dive into the research topics of 'Investigation of peroxynitrite induced oxidative stress in red blood cells monitored by luminol-dependent chemiluminescence'. Together they form a unique fingerprint.

Cite this