Investigation of peroxynitrite induced oxidative stress in red blood cells monitored by luminol-dependent chemiluminescence

Jaroslaw Kanski, Tanuja Koppal, D. Allan Butterfield

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Peroxynitrite-induced oxidative stress was investigated in red blood cells by luminol-dependent chemiluminescence. Red blood cells are susceptible to free radical damage due to the abundance of oxygen, presence of iron and other agents such as polyunsaturated fatty acids. Peroxynitrite (ONOO-), formed by the reaction of superoxide radical with nitric oxide radical, has been shown to cause protein and lipid oxidation. Addition of ONOO- showed a dramatic increase in chemiluminescence, but treatment with glutathione ethyl ester, an antioxidant, partially reduced the increase in chemiluminescence. The results show that red blood cells are a good model to mimic oxidative stress conditions and are discussed with relevance to neurodegenerate disorders.

Original languageEnglish
Pages (from-to)1183-1192
Number of pages10
JournalAnalytical Letters
Issue number6
StatePublished - 1999

Bibliographical note

Funding Information:
ACKNOWLEDGEMENTS This work was supported in part by NIH grants (AG-10836; AG-05119). The authors would like to express sincere gratitude to the persons who volunteered to donate their blood for the experiment and to Dr. Sylvia Daunert for use of the luminometer.


  • Chemiluminescence
  • Glutathione (GSH)
  • Luminol
  • Oxidative Stress (ROS)
  • Peroxynitrite
  • Red Blood Cells

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Spectroscopy
  • Clinical Biochemistry
  • Biochemistry, medical
  • Electrochemistry


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