TY - JOUR
T1 - Involvement of 5'-flanking κB-like sites within bcl-x gene in silica- induced Bcl-x expression
AU - Chen, Fei
AU - Demers, Laurence M.
AU - Vallyathan, Val
AU - Lu, Yongju
AU - Castranova, Vincent
AU - Shi, Xianglin
PY - 1999/12/10
Y1 - 1999/12/10
N2 - The present study investigated the involvement of the transcription factor NF-κB in the expression of an anti-apoptotic gene, bcl-x, using a murine macrophage cell line and peritoneal macrophages from both wild type (p50(+/+)) and NF-κB p50 gene knockout (p50(-/-)) mice. Increased expression of Bcl-x protein was observed in native and silica-exposed p50(-/-) macrophages in which the NF-κB p65-containing complex was predominantly induced. Co-transfection experiment using a bcl-x promoter reporter construct and an expression vector for NF-κB p50 or p65 indicates that p65, but not p50, upregulates the promoter activity of the bcl-x gene. DNA sequence analysis revealed that there are several κB-like sites within the 5'- flanking region of the bcl-x gene. Electrophoretic mobility shift assay suggested differences in binding of the NF-κB complexes to these putative NF-κB binding sites of the bcl-x gene.
AB - The present study investigated the involvement of the transcription factor NF-κB in the expression of an anti-apoptotic gene, bcl-x, using a murine macrophage cell line and peritoneal macrophages from both wild type (p50(+/+)) and NF-κB p50 gene knockout (p50(-/-)) mice. Increased expression of Bcl-x protein was observed in native and silica-exposed p50(-/-) macrophages in which the NF-κB p65-containing complex was predominantly induced. Co-transfection experiment using a bcl-x promoter reporter construct and an expression vector for NF-κB p50 or p65 indicates that p65, but not p50, upregulates the promoter activity of the bcl-x gene. DNA sequence analysis revealed that there are several κB-like sites within the 5'- flanking region of the bcl-x gene. Electrophoretic mobility shift assay suggested differences in binding of the NF-κB complexes to these putative NF-κB binding sites of the bcl-x gene.
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U2 - 10.1074/jbc.274.50.35591
DO - 10.1074/jbc.274.50.35591
M3 - Article
C2 - 10585435
AN - SCOPUS:0033544846
SN - 0021-9258
VL - 274
SP - 35591
EP - 35595
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 50
ER -