Involvement of CD5 in Th1 and Th2 contactmediated rescue of anti-μ and ionomycin induced growth inhibition in a B cell lymphoma

Subramanian Muthukkumar, Subbarao Bondada

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


Cross-linking of membrane Ig receptors by anti-i antibodies (Ab) or treatment with ionomycin induced complete growth arrest and subsequent apoptotic cell death in an immature B cell lymphoma, BKS-2. The growth-inhibitory signals delivered by anti-μ and ionomycin were overcome by anti-CD3-activated Th2 clones D10.G4 and F1 and by Th1 cell clone S53. In this report the Th-mediated growth reversal in BKS-2 cells was shown to require contact-dependent interactions when the inhibition was caused by immobilized anti-μ or ionomycin. Th2 cells in transwells (lymphokines) failed to protect BKS-2 cells from the growth-inhibitory effect of immobilized anti-μ or Ionomycin. Monoclonal antibodies to CD5 or CD40 llgands on activated Th cells partially inhibited the Th2 contact-dependent growth reversal of BKS-2 cells whereas simultaneous addition of both antibodies effectively prevented the delivery of contact-mediated growth signal. In contrast, anti-class I or class II Ab did not affect Th cell mediated growth reversal of BKS-2 cells. These data demonstrated that noncognate physical interaction with Th cells was essential for the recovery of BKS-2 cells when the latter were growth-arrested by strong inhibitory stimull such as immobilized anti-μ and ionomycin. Further CD5 as well as CD40 llgands on Th cells are important for signal transductlon in this type of T-B interaction.

Original languageEnglish
Pages (from-to)305-315
Number of pages11
JournalInternational Immunology
Issue number2
StatePublished - Feb 1995

Bibliographical note

Funding Information:
We thank Drs D. A. Cohen and E C Snow for critically reviewing this manuscript. This work was supported in part by grants Al 21490 and AG 05731, and a career research development award K04 AG 00422 (to S. B.) from the National Institutes of Health.


  • Anti-μ
  • CD5
  • Growth reversal
  • Ionomycin
  • Th contact-mediated signal

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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