Involvement of phospholipase D in wound-induced accumulation of jasmonic acid in Arabidopsis

C. Wang, C. A. Zien, M. Afitlhile, R. Welti, D. F. Hildebrand, X. Wang

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229 Scopus citations


Multiple forms of phospholipase D (PLD) were activated in response to wounding, and the expressions of PLDα, PLDβ, and PLDγ differed in wounded Arabidopsis leaves. Antisense abrogation of the common plant PLD, PLDα, decreased the wound induction of phosphatidic acid, jasmonic acid (JA), and a JA-regulated gene for vegetative storage protein. Examination of the genes involved in the initial steps of oxylipin synthesis revealed that abrogation of the PLDα attenuated the wound-induced expression of lipoxygenase 2 (LOX2) but had no effect on allene oxide synthase (AOS) or hydroperoxide lyase in wounded leaves. The systemic induction of LOX2, AOS, and vegetative storage protein was lower in the PLDα-suppressed plants than in wild-type plants, with AOS exhibiting a distinct pattern. These results indicate that activation of PLD mediates wound induction of JA and that LOX2 is probably a downstream target through which PLD promotes the production of JA.

Original languageEnglish
Pages (from-to)2237-2246
Number of pages10
JournalPlant Cell
Issue number11
StatePublished - 2000

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology


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