Experimental autoimmune uveitis (EAU) is a Th1-cell-mediated autoimmune disease. In this study, the correlation between IRBP-specific Th1 cells in PBLs and the histological grading in the eyes was evaluated kinetically during EAU induction. EAU was induced in B10.A mice with IRBP immunization and the eyes were enucleated for histological examination on days 0, 3, 7, 15, and 21 after immunization. To determine the Th1-cell-mediated immune response, Th1 cytokines (IL-12p40 and IFN-γ) were measured by RT-PCR in inflamed eyes. At mean time, CD4+ and IFN-γ+ double positive T cells (Th1 cells) from PBLs were analyzed by flow cytometry. The level of the IRBP-specific Th1 cells was significantly increased and kinetically changed during EAU induction, but the cells reached peak time early before the disease was onset. Those IRBP-specific Th1 cells in the PBLs were evidence for EAU disease, but its peak time was different from EAU disease in the eyes. Our data suggested that it is very important to collect blood from patients at a suitable time point and the Th1 cells measured by flow cytometry are good marker for disease diagnosis.
|Number of pages||6|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Feb 28 2003|
Bibliographical noteFunding Information:
We are grateful to Dr. Ye Ming for reviewing the manuscript and for helpful comments. This work was supported by 973 project (G1999053907), National Natural Science Foundation of China (30070708), and key Projects of Chinese Academy of Sciences (KSCX2-2-08-02) and National Key Basic Research Program of China (2001CB510006).
- Experimental autoimmune uveitis
- Flow cytometry
- Peripheral blood lymphocytes
- Th1 cell
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology