Isolation and characterization of mutants of Kluyveromyces lactis defective in lactose transport

Michael I. Riley, K. Sreekrishna, Srirama Bhairi, Robert C. Dickson

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

Mutants of Kluyveromyces lactis defective in lactose transport were identified among lactose-resistant revertants of lactose-sensitive strains. The mutations are closely linked to the β-galactosidase gene, LAC4, and they are located in a previously identified gene, LAC12, which has been shown to code for a lactose permease. Our data establish that LAC12 is the only lactose permease gene in K. lactis. The lactose permease also transports galactose. LAC12 is transcribed in a direction opposite to that of LAC4, there being about 2.5 kb between their transcription start sites. Transcription of LAC12 is inducible as is that of all other structural genes in the lactose-galactose regulon of K. lactis.

Original languageEnglish
Pages (from-to)145-151
Number of pages7
JournalMGG Molecular & General Genetics
Volume208
Issue number1-2
DOIs
StatePublished - Jun 1987

Keywords

  • LAC12
  • Permease
  • Yeast

ASJC Scopus subject areas

  • Genetics

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