Isolation and development of bovine primary respiratory cells as model to study influenza D virus infection

Tirth Uprety; Chithra C. Sreenivasan; Shaurav Bhattarai; Dan Wang; Radhey S. Kaushik; Feng Li

doi:10.1016/j.virol.2021.04.003

Isolation and development of bovine primary respiratory cells as model to study influenza D virus infection

Tirth Uprety, Chithra C. Sreenivasan, Shaurav Bhattarai, Dan Wang, Radhey S. Kaushik, Feng Li

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Influenza D virus (IDV) is a novel type of influenza virus that infects and causes respiratory illness in bovines. Lack of host-specific in vitro model that can recapitulate morphology and physiology of in vivo airway epithelial cells has impeded the study of IDV infection. Here, we established and characterized bovine primary respiratory epithelial cells from nasal turbinate, soft palate, and trachea of the same calf. All three cell types showed characteristics peculiar of epithelial cells, polarized into apical-basolateral membrane, and formed tight junctions. Furthermore, these cells expressed both α-2,3- and α-2,6-linked sialic acids with α-2,3 linkage being more abundant. IDV strains replicated to high titers in these cells, while influenza A and B viruses exhibited moderate to low titers, with influenza C virus replication not detected. These findings suggest that bovine primary airway epithelial cells can be utilized to model infection biology and pathophysiology of IDV and other respiratory pathogens.

Original language	English
Pages (from-to)	89-99
Number of pages	11
Journal	Virology
Volume	559
DOIs	https://doi.org/10.1016/j.virol.2021.04.003
State	Published - Jul 2021

Bibliographical note

Publisher Copyright:
© 2021 Elsevier Inc.

Funding

This study was supported by NIH R01AI141889 , Agricultural Experiment Station of the University of Kentucky , the William Robert Mills Chair Endowment Fund , and SDSU Agricultural Experiment Station Hatch grants # SD00H701-20 and 3AH-673 . We would also like to acknowledge the SDSU meat lab for providing tissue samples.

Funders	Funder number
National Institutes of Health (NIH)
National Institute of Allergy and Infectious Diseases	R01AI141889
San Diego State University	SD00H701-20, 3AH-673
University of Kentucky

Keywords

Bovine
Influenza D virus
Nasal turbinate
Primary cells
Respiratory epithelial cells
Soft palate
Trachea

ASJC Scopus subject areas

Virology

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10.1016/j.virol.2021.04.003

Cite this

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title = "Isolation and development of bovine primary respiratory cells as model to study influenza D virus infection",

abstract = "Influenza D virus (IDV) is a novel type of influenza virus that infects and causes respiratory illness in bovines. Lack of host-specific in vitro model that can recapitulate morphology and physiology of in vivo airway epithelial cells has impeded the study of IDV infection. Here, we established and characterized bovine primary respiratory epithelial cells from nasal turbinate, soft palate, and trachea of the same calf. All three cell types showed characteristics peculiar of epithelial cells, polarized into apical-basolateral membrane, and formed tight junctions. Furthermore, these cells expressed both α-2,3- and α-2,6-linked sialic acids with α-2,3 linkage being more abundant. IDV strains replicated to high titers in these cells, while influenza A and B viruses exhibited moderate to low titers, with influenza C virus replication not detected. These findings suggest that bovine primary airway epithelial cells can be utilized to model infection biology and pathophysiology of IDV and other respiratory pathogens.",

keywords = "Bovine, Influenza D virus, Nasal turbinate, Primary cells, Respiratory epithelial cells, Soft palate, Trachea",

author = "Tirth Uprety and Sreenivasan, {Chithra C.} and Shaurav Bhattarai and Dan Wang and Kaushik, {Radhey S.} and Feng Li",

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year = "2021",

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doi = "10.1016/j.virol.2021.04.003",

language = "English",

volume = "559",

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AU - Uprety, Tirth

AU - Sreenivasan, Chithra C.

AU - Bhattarai, Shaurav

AU - Wang, Dan

AU - Kaushik, Radhey S.

AU - Li, Feng

PY - 2021/7

Y1 - 2021/7

N2 - Influenza D virus (IDV) is a novel type of influenza virus that infects and causes respiratory illness in bovines. Lack of host-specific in vitro model that can recapitulate morphology and physiology of in vivo airway epithelial cells has impeded the study of IDV infection. Here, we established and characterized bovine primary respiratory epithelial cells from nasal turbinate, soft palate, and trachea of the same calf. All three cell types showed characteristics peculiar of epithelial cells, polarized into apical-basolateral membrane, and formed tight junctions. Furthermore, these cells expressed both α-2,3- and α-2,6-linked sialic acids with α-2,3 linkage being more abundant. IDV strains replicated to high titers in these cells, while influenza A and B viruses exhibited moderate to low titers, with influenza C virus replication not detected. These findings suggest that bovine primary airway epithelial cells can be utilized to model infection biology and pathophysiology of IDV and other respiratory pathogens.

AB - Influenza D virus (IDV) is a novel type of influenza virus that infects and causes respiratory illness in bovines. Lack of host-specific in vitro model that can recapitulate morphology and physiology of in vivo airway epithelial cells has impeded the study of IDV infection. Here, we established and characterized bovine primary respiratory epithelial cells from nasal turbinate, soft palate, and trachea of the same calf. All three cell types showed characteristics peculiar of epithelial cells, polarized into apical-basolateral membrane, and formed tight junctions. Furthermore, these cells expressed both α-2,3- and α-2,6-linked sialic acids with α-2,3 linkage being more abundant. IDV strains replicated to high titers in these cells, while influenza A and B viruses exhibited moderate to low titers, with influenza C virus replication not detected. These findings suggest that bovine primary airway epithelial cells can be utilized to model infection biology and pathophysiology of IDV and other respiratory pathogens.

KW - Bovine

KW - Influenza D virus

KW - Nasal turbinate

KW - Primary cells

KW - Respiratory epithelial cells

KW - Soft palate

KW - Trachea

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Isolation and development of bovine primary respiratory cells as model to study influenza D virus infection

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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