We have isolated and characterized a new series of B lymphomas which occurred spontaneously in a group of CBA N mice that were transferred with spleen or lymph node cells from 24-month-old CBA Ca mice. Tumor cell lines from six CBA N mice that received spleen cells were rescued and designated as BKS-2, BKS-3, BKS-4, BKS-5, BKS-6, and BKS-7. Also, tumor cells from a recipient of lymph node cells were rescued and the resulting cell line was designated BKL. These tumor cells expressed membrane immunoglobulin (μ,κ), major histocompatibility complex Class I and Class II molecules, B220, Lyb8, Fc receptors, J11d, interleukin 2 receptors, and Ly1. All of the tumors did not express the T cell specific markers Thy 1.2, L3T4, and Lyt2.1. They appeared to be clonal in origin, since they exhibited common rearrangements at both heavy and light chain immunoglobulin loci. Phenotypically, these lymphomas appeared to be analogous to immature B cells. Also, these lymphomas displayed different functional reactivities when treated with various B cell mitogens and growth factors in vitro. Anti-μ antibodies which normally induce B cell growth inhibited the proliferation of these lymphoma cells in vitro, whereas they responded to lipopolysaccharide, T cell-derived growth factors, and interleukin 5 by enhanced proliferation. These tumor cells expressed constitutively high levels of c-myc mRNA.
|Number of pages||12|
|Journal||Clinical Immunology and Immunopathology|
|State||Published - May 1989|
Bibliographical noteFunding Information:
We are grateful to Mrs. Ela S. Pate1 for performing histology work and Drs. William R. Markesbery and Michael L. Cibull for histopathological identification of tumors. We thank Ms. Catherine Nowack for her help in the FACS system and Mrs. Charlotte Burchett for typing this manuscript. We also acknowledge Dr. Henry H. Wortis for critically reviewing this manuscript. This work was supported in part by Grants AI21490 and AG-05731, and V.U. was supported in part by a postdoctoral fellowship from the PSP funds of the University of Kentucky. B.S. is a recipient of Career Research Development Award K04 AGO0422 from the National Institute of Health.
ASJC Scopus subject areas
- Immunology and Allergy
- Pathology and Forensic Medicine