Isolation and molecular characterization of the 5'-upstream region of the human TRAIL gene

Qingding Wang; Yanshan Ji; Xiaofu Wang; B. Mark Evers

doi:10.1006/bbrc.2000.3512

Isolation and molecular characterization of the 5'-upstream region of the human TRAIL gene

Qingding Wang, Yanshan Ji, Xiaofu Wang, B. Mark Evers

Research output: Contribution to journal › Article › peer-review

91 Scopus citations

Abstract

TRAIL, a novel member of the TNF family, acts through membrane receptors to induce apoptosis of activated T lymphocytes and may represent a mechanism for the 'immune escape' of certain cancers. Various cytokines appear to increase expression of other TNF family members; however, the regulation of TRAIL has not been defined. The purpose of this study was to assess molecular mechanisms regulating TRAIL gene expression in human colon cancers. In this study, we have cloned the human TRAIL (hTRAIL) promoter (~1.6 kb) and identified a number of putative transcription factor binding sites such as NFAT, AP-1 and Sp1 sequences which are important for the expression of other TNF family members. Transient transfections of 5'-deletion promoter constructs into either Caco-2 or HT29 colon cancer cells identified TRAIL promoter regions critical for both basal and interferon-γ (IFN-γ)-mediated induction. Furthermore, induction of TRAIL mRNA levels was demonstrated in HT29 and Caco-2 cells with IFN-γ treatment suggesting an important role for this cytokine in TRAIL expression. (C) 2000 Academic Press.

Original language	English
Pages (from-to)	466-471
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	276
Issue number	2
DOIs	https://doi.org/10.1006/bbrc.2000.3512
State	Published - Sep 24 2000

Bibliographical note

Funding Information:
The authors thank Eileen Figueroa and Karen Martin for preparation of the manuscript. This work was funded by grants from the National Institutes of Health (R01 DK48498, R01 AG10885, P01 DK35608).

Funding

The authors thank Eileen Figueroa and Karen Martin for preparation of the manuscript. This work was funded by grants from the National Institutes of Health (R01 DK48498, R01 AG10885, P01 DK35608).

Funders	Funder number
National Institutes of Health (NIH)	P01 DK35608, R01 AG10885
National Institute of Diabetes and Digestive and Kidney Diseases	R01DK048498

Keywords

Caco-2
HT29
Human colon cancers
IFN-γ
TNF family
TRAIL

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1006/bbrc.2000.3512

Cite this

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title = "Isolation and molecular characterization of the 5'-upstream region of the human TRAIL gene",

abstract = "TRAIL, a novel member of the TNF family, acts through membrane receptors to induce apoptosis of activated T lymphocytes and may represent a mechanism for the 'immune escape' of certain cancers. Various cytokines appear to increase expression of other TNF family members; however, the regulation of TRAIL has not been defined. The purpose of this study was to assess molecular mechanisms regulating TRAIL gene expression in human colon cancers. In this study, we have cloned the human TRAIL (hTRAIL) promoter (~1.6 kb) and identified a number of putative transcription factor binding sites such as NFAT, AP-1 and Sp1 sequences which are important for the expression of other TNF family members. Transient transfections of 5'-deletion promoter constructs into either Caco-2 or HT29 colon cancer cells identified TRAIL promoter regions critical for both basal and interferon-γ (IFN-γ)-mediated induction. Furthermore, induction of TRAIL mRNA levels was demonstrated in HT29 and Caco-2 cells with IFN-γ treatment suggesting an important role for this cytokine in TRAIL expression. (C) 2000 Academic Press.",

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author = "Qingding Wang and Yanshan Ji and Xiaofu Wang and Evers, {B. Mark}",

note = "Funding Information: The authors thank Eileen Figueroa and Karen Martin for preparation of the manuscript. This work was funded by grants from the National Institutes of Health (R01 DK48498, R01 AG10885, P01 DK35608).",

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AU - Wang, Qingding

AU - Ji, Yanshan

AU - Wang, Xiaofu

AU - Evers, B. Mark

N1 - Funding Information: The authors thank Eileen Figueroa and Karen Martin for preparation of the manuscript. This work was funded by grants from the National Institutes of Health (R01 DK48498, R01 AG10885, P01 DK35608).

PY - 2000/9/24

Y1 - 2000/9/24

N2 - TRAIL, a novel member of the TNF family, acts through membrane receptors to induce apoptosis of activated T lymphocytes and may represent a mechanism for the 'immune escape' of certain cancers. Various cytokines appear to increase expression of other TNF family members; however, the regulation of TRAIL has not been defined. The purpose of this study was to assess molecular mechanisms regulating TRAIL gene expression in human colon cancers. In this study, we have cloned the human TRAIL (hTRAIL) promoter (~1.6 kb) and identified a number of putative transcription factor binding sites such as NFAT, AP-1 and Sp1 sequences which are important for the expression of other TNF family members. Transient transfections of 5'-deletion promoter constructs into either Caco-2 or HT29 colon cancer cells identified TRAIL promoter regions critical for both basal and interferon-γ (IFN-γ)-mediated induction. Furthermore, induction of TRAIL mRNA levels was demonstrated in HT29 and Caco-2 cells with IFN-γ treatment suggesting an important role for this cytokine in TRAIL expression. (C) 2000 Academic Press.

AB - TRAIL, a novel member of the TNF family, acts through membrane receptors to induce apoptosis of activated T lymphocytes and may represent a mechanism for the 'immune escape' of certain cancers. Various cytokines appear to increase expression of other TNF family members; however, the regulation of TRAIL has not been defined. The purpose of this study was to assess molecular mechanisms regulating TRAIL gene expression in human colon cancers. In this study, we have cloned the human TRAIL (hTRAIL) promoter (~1.6 kb) and identified a number of putative transcription factor binding sites such as NFAT, AP-1 and Sp1 sequences which are important for the expression of other TNF family members. Transient transfections of 5'-deletion promoter constructs into either Caco-2 or HT29 colon cancer cells identified TRAIL promoter regions critical for both basal and interferon-γ (IFN-γ)-mediated induction. Furthermore, induction of TRAIL mRNA levels was demonstrated in HT29 and Caco-2 cells with IFN-γ treatment suggesting an important role for this cytokine in TRAIL expression. (C) 2000 Academic Press.

KW - Caco-2

KW - HT29

KW - Human colon cancers

KW - IFN-γ

KW - TNF family

KW - TRAIL

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Isolation and molecular characterization of the 5'-upstream region of the human TRAIL gene

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

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