Isolation and physical characterization of three essential conidiation genes from Aspergillusnidulans

William E. Timberlake, Margaret T. Boylan, Peter M. Mirabito, Catherine E. Willett, Charles R. Zimmerman

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


We cloned and characterized three genes from Aspergillusnidulans, designated brlA, abaA and wetA, whose activities are required to complete different stages of conidiophore development. Inactivation of these genes causes major abnormalities in conidiophore morphology and prevents expression of many unrelated, developmentally regulated genes, without affecting expression of nonregulated genes. The three genes code for poly(A)+RNAs that begin to accumulate at different times during conidiation. The brlA-and abaA-encoded RNAs accumulate specifically in cells of the conidiophore. The wetA-encoded RNA accumulates in mature conidia. Inactivation of the brlA gene prevents expression of the abaA and wetA genes, whereas inactivation of the abaA gene prevents expression of the wetA gene. Our results confirm genetic predictions as to the temporal and spatial patterns of expression of these genes and demonstrate that these patterns are specified at the level of RNA accumulation.

Original languageEnglish
Pages (from-to)317
Number of pages1
JournalAntonie van Leeuwenhoek
Issue number5
StatePublished - Sep 1987

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology


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