Isolation of neural precursor cells from Alzheimer's disease and aged control postmortem brain

Mark A. Lovell; Hartmut Geiger; Gary E. Van Zant; Bert C. Lynn; William R. Markesbery

doi:10.1016/j.neurobiolaging.2005.05.004

Isolation of neural precursor cells from Alzheimer's disease and aged control postmortem brain

Mark A. Lovell, Hartmut Geiger, Gary E. Van Zant, Bert C. Lynn, William R. Markesbery

Research output: Contribution to journal › Article › peer-review

49 Scopus citations

Abstract

Recent studies demonstrate that isolated neural precursor cells are capable of generating neurons, astrocytes, and oligodendrocytes from neurogenic regions of adult brain. Because these studies use surgically resected or fresh postmortem specimens from young subjects, it is not clear whether neural precursor cells remain in the brain of normal aged subjects or subjects with Alzheimer's disease (AD). The purpose of this study was to determine if viable precursor cells remain in aged control and AD brain. AD subjects have significantly fewer viable precursor cells in the hippocampus compared with age-matched normal control subjects. Musashi-1 and Ki-67-positive precursor cells from AD self renew, but reach senescence earlier than cells isolated from normal aged control subjects. Precursor cells from AD and aged normal control specimens can differentiate into tubulin- and Tuj-1-positive neurons and GFAP-positive astrocytes. This study demonstrates that viable precursor cells remain in AD and aged normal control brain specimens and can be induced to differentiate. These results raise the possibility of stimulation of inherent precursor cells of aged individuals or AD patients to replace neurons lost in aging and/or neurodegeneration.

Original language	English
Pages (from-to)	909-917
Number of pages	9
Journal	Neurobiology of Aging
Volume	27
Issue number	7
DOIs	https://doi.org/10.1016/j.neurobiolaging.2005.05.004
State	Published - Jul 2006

Bibliographical note

Funding Information:
This work was supported by National Institutes of Health Grants 5P50-AG05144, 5P01-AG05119 and grants from the Abercrombie Foundation and Kleberg Foundation. The authors thank Chengsong Xie and Shuling Xiong for assistance with Western blots and micrographs, Paula Thomason for editorial assistance, and Sonya Anderson for subject demographic data.

Funding

This work was supported by National Institutes of Health Grants 5P50-AG05144, 5P01-AG05119 and grants from the Abercrombie Foundation and Kleberg Foundation. The authors thank Chengsong Xie and Shuling Xiong for assistance with Western blots and micrographs, Paula Thomason for editorial assistance, and Sonya Anderson for subject demographic data.

Funders	Funder number
National Institutes of Health (NIH)	5P50-AG05144
National Institute on Aging	P01AG005119
Abercrombie Foundation
Robert J. Kleberg, Jr. and Helen C. Kleberg Foundation

Keywords

Alzheimer's disease
Neural stem/precursor cells
Neurodegeneration

ASJC Scopus subject areas

General Neuroscience
Aging
Clinical Neurology
Developmental Biology
Geriatrics and Gerontology

Access to Document

10.1016/j.neurobiolaging.2005.05.004

Cite this

@article{5aefbaa1d3a048319633d30246871edf,

title = "Isolation of neural precursor cells from Alzheimer's disease and aged control postmortem brain",

abstract = "Recent studies demonstrate that isolated neural precursor cells are capable of generating neurons, astrocytes, and oligodendrocytes from neurogenic regions of adult brain. Because these studies use surgically resected or fresh postmortem specimens from young subjects, it is not clear whether neural precursor cells remain in the brain of normal aged subjects or subjects with Alzheimer's disease (AD). The purpose of this study was to determine if viable precursor cells remain in aged control and AD brain. AD subjects have significantly fewer viable precursor cells in the hippocampus compared with age-matched normal control subjects. Musashi-1 and Ki-67-positive precursor cells from AD self renew, but reach senescence earlier than cells isolated from normal aged control subjects. Precursor cells from AD and aged normal control specimens can differentiate into tubulin- and Tuj-1-positive neurons and GFAP-positive astrocytes. This study demonstrates that viable precursor cells remain in AD and aged normal control brain specimens and can be induced to differentiate. These results raise the possibility of stimulation of inherent precursor cells of aged individuals or AD patients to replace neurons lost in aging and/or neurodegeneration.",

keywords = "Alzheimer's disease, Neural stem/precursor cells, Neurodegeneration",

author = "Lovell, {Mark A.} and Hartmut Geiger and {Van Zant}, {Gary E.} and Lynn, {Bert C.} and Markesbery, {William R.}",

note = "Funding Information: This work was supported by National Institutes of Health Grants 5P50-AG05144, 5P01-AG05119 and grants from the Abercrombie Foundation and Kleberg Foundation. The authors thank Chengsong Xie and Shuling Xiong for assistance with Western blots and micrographs, Paula Thomason for editorial assistance, and Sonya Anderson for subject demographic data.",

year = "2006",

month = jul,

doi = "10.1016/j.neurobiolaging.2005.05.004",

language = "English",

volume = "27",

pages = "909--917",

number = "7",

}

TY - JOUR

T1 - Isolation of neural precursor cells from Alzheimer's disease and aged control postmortem brain

AU - Lovell, Mark A.

AU - Geiger, Hartmut

AU - Van Zant, Gary E.

AU - Lynn, Bert C.

AU - Markesbery, William R.

N1 - Funding Information: This work was supported by National Institutes of Health Grants 5P50-AG05144, 5P01-AG05119 and grants from the Abercrombie Foundation and Kleberg Foundation. The authors thank Chengsong Xie and Shuling Xiong for assistance with Western blots and micrographs, Paula Thomason for editorial assistance, and Sonya Anderson for subject demographic data.

PY - 2006/7

Y1 - 2006/7

N2 - Recent studies demonstrate that isolated neural precursor cells are capable of generating neurons, astrocytes, and oligodendrocytes from neurogenic regions of adult brain. Because these studies use surgically resected or fresh postmortem specimens from young subjects, it is not clear whether neural precursor cells remain in the brain of normal aged subjects or subjects with Alzheimer's disease (AD). The purpose of this study was to determine if viable precursor cells remain in aged control and AD brain. AD subjects have significantly fewer viable precursor cells in the hippocampus compared with age-matched normal control subjects. Musashi-1 and Ki-67-positive precursor cells from AD self renew, but reach senescence earlier than cells isolated from normal aged control subjects. Precursor cells from AD and aged normal control specimens can differentiate into tubulin- and Tuj-1-positive neurons and GFAP-positive astrocytes. This study demonstrates that viable precursor cells remain in AD and aged normal control brain specimens and can be induced to differentiate. These results raise the possibility of stimulation of inherent precursor cells of aged individuals or AD patients to replace neurons lost in aging and/or neurodegeneration.

AB - Recent studies demonstrate that isolated neural precursor cells are capable of generating neurons, astrocytes, and oligodendrocytes from neurogenic regions of adult brain. Because these studies use surgically resected or fresh postmortem specimens from young subjects, it is not clear whether neural precursor cells remain in the brain of normal aged subjects or subjects with Alzheimer's disease (AD). The purpose of this study was to determine if viable precursor cells remain in aged control and AD brain. AD subjects have significantly fewer viable precursor cells in the hippocampus compared with age-matched normal control subjects. Musashi-1 and Ki-67-positive precursor cells from AD self renew, but reach senescence earlier than cells isolated from normal aged control subjects. Precursor cells from AD and aged normal control specimens can differentiate into tubulin- and Tuj-1-positive neurons and GFAP-positive astrocytes. This study demonstrates that viable precursor cells remain in AD and aged normal control brain specimens and can be induced to differentiate. These results raise the possibility of stimulation of inherent precursor cells of aged individuals or AD patients to replace neurons lost in aging and/or neurodegeneration.

KW - Alzheimer's disease

KW - Neural stem/precursor cells

KW - Neurodegeneration

UR - http://www.scopus.com/inward/record.url?scp=33746418611&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33746418611&partnerID=8YFLogxK

U2 - 10.1016/j.neurobiolaging.2005.05.004

DO - 10.1016/j.neurobiolaging.2005.05.004

M3 - Article

C2 - 15979211

AN - SCOPUS:33746418611

SN - 0197-4580

VL - 27

SP - 909

EP - 917

JO - Neurobiology of Aging

JF - Neurobiology of Aging

IS - 7

ER -

Isolation of neural precursor cells from Alzheimer's disease and aged control postmortem brain

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this