Isolation of Rhp-PSP, a member of YER057c/YjgF/UK114 protein family with antiviral properties, from the photosynthetic bacterium Rhodopseudomonas palustris strain JSC-3b

Pin Su; Tuizi Feng; Xuguo Zhou; Songbai Zhang; Yu Zhang; Ju'e Cheng; Yuanhua Luo; Jing Peng; Zhuo Zhang; Xiangyang Lu; Deyong Zhang; Yong Liu

doi:10.1038/srep16121

Isolation of Rhp-PSP, a member of YER057c/YjgF/UK114 protein family with antiviral properties, from the photosynthetic bacterium Rhodopseudomonas palustris strain JSC-3b

Pin Su, Tuizi Feng, Xuguo Zhou, Songbai Zhang, Yu Zhang, Ju'e Cheng, Yuanhua Luo, Jing Peng, Zhuo Zhang, Xiangyang Lu, Deyong Zhang, Yong Liu

Entomology

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Abstract

Rhodopseudomonas palustris strain JSC-3b isolated from a water canal adjacent to a vegetable field produces a protein that was purified by bioactivity-guided fractionation based on ammonium sulfate precipitation, ion-exchange absorption and size exclusion. The protein was further identified as an endoribonuclease L-PSP (Liver-Perchloric acid-soluble protein) by shotgun mass spectrometry analysis and gene identification, and it is member of YER057c/YjgF/UK114 protein family. Herein, this protein is designated Rhp-PSP. Rhp-PSP exhibited significant inhibitory activities against tobacco mosaic virus (TMV) in vivo and in vitro. To our knowledge, this represents the first report on the antiviral activity of a protein of the YER057c/YjgF/UK114 family and also the first antiviral protein isolated from R. palustris. Our research provides insight into the potential of photosynthetic bacterial resources in biological control of plant virus diseases and sustainable agriculture.

Original language	English
Article number	16121
Journal	Scientific Reports
Volume	5
DOIs	https://doi.org/10.1038/srep16121
State	Published - Nov 4 2015

Bibliographical note

Funding Information:
This research was supported by the Special Fund for the Agro-scientific Research in the Public Interest (201303028), the National Science Foundation of China (31272067) and the Agricultural Research System of China (CARS-25-B-05). The grant agencies had no role in study design, data collection and analysis, the decision to publish, or the preparation of this manuscript.

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Su, P., Feng, T., Zhou, X., Zhang, S., Zhang, Y., Cheng, J., Luo, Y., Peng, J., Zhang, Z., Lu, X., Zhang, D., & Liu, Y. (2015). Isolation of Rhp-PSP, a member of YER057c/YjgF/UK114 protein family with antiviral properties, from the photosynthetic bacterium Rhodopseudomonas palustris strain JSC-3b. Scientific Reports, 5, Article 16121. https://doi.org/10.1038/srep16121

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title = "Isolation of Rhp-PSP, a member of YER057c/YjgF/UK114 protein family with antiviral properties, from the photosynthetic bacterium Rhodopseudomonas palustris strain JSC-3b",

abstract = "Rhodopseudomonas palustris strain JSC-3b isolated from a water canal adjacent to a vegetable field produces a protein that was purified by bioactivity-guided fractionation based on ammonium sulfate precipitation, ion-exchange absorption and size exclusion. The protein was further identified as an endoribonuclease L-PSP (Liver-Perchloric acid-soluble protein) by shotgun mass spectrometry analysis and gene identification, and it is member of YER057c/YjgF/UK114 protein family. Herein, this protein is designated Rhp-PSP. Rhp-PSP exhibited significant inhibitory activities against tobacco mosaic virus (TMV) in vivo and in vitro. To our knowledge, this represents the first report on the antiviral activity of a protein of the YER057c/YjgF/UK114 family and also the first antiviral protein isolated from R. palustris. Our research provides insight into the potential of photosynthetic bacterial resources in biological control of plant virus diseases and sustainable agriculture.",

author = "Pin Su and Tuizi Feng and Xuguo Zhou and Songbai Zhang and Yu Zhang and Ju'e Cheng and Yuanhua Luo and Jing Peng and Zhuo Zhang and Xiangyang Lu and Deyong Zhang and Yong Liu",

note = "Funding Information: This research was supported by the Special Fund for the Agro-scientific Research in the Public Interest (201303028), the National Science Foundation of China (31272067) and the Agricultural Research System of China (CARS-25-B-05). The grant agencies had no role in study design, data collection and analysis, the decision to publish, or the preparation of this manuscript.",

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AU - Su, Pin

AU - Feng, Tuizi

AU - Zhou, Xuguo

AU - Zhang, Songbai

AU - Zhang, Yu

AU - Cheng, Ju'e

AU - Luo, Yuanhua

AU - Peng, Jing

AU - Zhang, Zhuo

AU - Lu, Xiangyang

AU - Zhang, Deyong

AU - Liu, Yong

N1 - Funding Information: This research was supported by the Special Fund for the Agro-scientific Research in the Public Interest (201303028), the National Science Foundation of China (31272067) and the Agricultural Research System of China (CARS-25-B-05). The grant agencies had no role in study design, data collection and analysis, the decision to publish, or the preparation of this manuscript.

PY - 2015/11/4

Y1 - 2015/11/4

N2 - Rhodopseudomonas palustris strain JSC-3b isolated from a water canal adjacent to a vegetable field produces a protein that was purified by bioactivity-guided fractionation based on ammonium sulfate precipitation, ion-exchange absorption and size exclusion. The protein was further identified as an endoribonuclease L-PSP (Liver-Perchloric acid-soluble protein) by shotgun mass spectrometry analysis and gene identification, and it is member of YER057c/YjgF/UK114 protein family. Herein, this protein is designated Rhp-PSP. Rhp-PSP exhibited significant inhibitory activities against tobacco mosaic virus (TMV) in vivo and in vitro. To our knowledge, this represents the first report on the antiviral activity of a protein of the YER057c/YjgF/UK114 family and also the first antiviral protein isolated from R. palustris. Our research provides insight into the potential of photosynthetic bacterial resources in biological control of plant virus diseases and sustainable agriculture.

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Isolation of Rhp-PSP, a member of YER057c/YjgF/UK114 protein family with antiviral properties, from the photosynthetic bacterium Rhodopseudomonas palustris strain JSC-3b

Abstract

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