Isolation of the Arabidopsis ABI3 gene by positional cloning

Jérôme Giraudat, Brian M. Hauge, Christiane Valon, Jan Smalle, François Parcy, Howard M. Goodman

Research output: Contribution to journalArticlepeer-review

894 Scopus citations

Abstract

Arabidopsis abi3 mutants are altered in various aspects of seed development and germination that reflect a decreased responsiveness to the hormone abscisic acid. The ABI3 gene has been isolated by positional cloning. A detailed restriction fragment length polymorphism (RFLP) map of the abi3 region was constructed. An RFLP marker closely linked to the abi3 locus was identified, and by analyzing an overlapping set of cosmid clones containing this marker, the abi3 locus was localized within a 35-kb region. An 11-kb subfragment was then shown to complement the mutant phenotype in transgenic plants, thereby further delimiting the position of the locus. A candidate ABI3 gene was identified within this fragment as being expressed in developing fruits. The primary structure of the encoded protein was deduced from sequence analysis of a corresponding cDNA clone. In the most severe abi3-4 allele, the size of this predicted protein was reduced by 40% due to the presence of a point mutation that introduced a premature stop codon. The predicted ABI3 protein displays discrete regions of high similarity to the maize viviparous-1 protein.

Original languageEnglish
Pages (from-to)1251-1261
Number of pages11
JournalPlant Cell
Volume4
Issue number10
DOIs
StatePublished - Oct 1992

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

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