Isotopomer-Based Metabolomic Analysis by NMR and Mass Spectrometry

Andrew N. Lane; Teresa W.M. Fan; Richard M. Higashi

doi:10.1016/S0091-679X(07)84018-0

Isotopomer-Based Metabolomic Analysis by NMR and Mass Spectrometry

Andrew N. Lane, Teresa W.M. Fan, Richard M. Higashi

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

103 Scopus citations

Abstract

Nuclear magnetic resonance (NMR) and mass spectrometry (MS) together are synergistic in their ability to profile comprehensively the metabolome of cells and tissues. In addition to identification and quantification of metabolites, changes in metabolic pathways and fluxes in response to external perturbations can be reliably determined by using stable isotope tracer methodologies. NMR and MS together are able to define both positional isotopomer distribution in product metabolites that derive from a given stable isotope-labeled precursor molecule and the degree of enrichment at each site with good precision. Together with modeling tools, this information provides a rich functional biochemical readout of cellular activity and how it responds to external influences. In this chapter, we describe NMR- and MS-based methodologies for isotopomer analysis in metabolomics and its applications for different biological systems.

Original language	English
Title of host publication	Biophysical Tools for Biologists, Volume One
Subtitle of host publication	In Vitro Techniques
Editors	John Correi, William Detrich, III
Pages	541-588
Number of pages	48
DOIs	https://doi.org/10.1016/S0091-679X(07)84018-0
State	Published - 2008

Publication series

Name	Methods in Cell Biology
Volume	84
ISSN (Print)	0091-679X

Bibliographical note

Funding Information:
This work was supported in part by grants from the Susan G. Komen Foundation Grant BCTR0503648, Kentucky Science and Engineering Foundation, KSEF‐296‐RDE‐3, NSF EPSCoR EPS‐0447479, NCI 1RO1 CA101199, and the Brown Foundation.

Funding

This work was supported in part by grants from the Susan G. Komen Foundation Grant BCTR0503648, Kentucky Science and Engineering Foundation, KSEF‐296‐RDE‐3, NSF EPSCoR EPS‐0447479, NCI 1RO1 CA101199, and the Brown Foundation.

Funders	Funder number
NSF EPSCoR EPS‐0447479
National Childhood Cancer Registry – National Cancer Institute	R01CA101199
Brown Foundation
Susan G Komen Foundation	BCTR0503648
Kentucky Science and Engineering Foundation	KSEF‐296‐RDE‐3

ASJC Scopus subject areas

Cell Biology

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10.1016/S0091-679X(07)84018-0

Cite this

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title = "Isotopomer-Based Metabolomic Analysis by NMR and Mass Spectrometry",

abstract = "Nuclear magnetic resonance (NMR) and mass spectrometry (MS) together are synergistic in their ability to profile comprehensively the metabolome of cells and tissues. In addition to identification and quantification of metabolites, changes in metabolic pathways and fluxes in response to external perturbations can be reliably determined by using stable isotope tracer methodologies. NMR and MS together are able to define both positional isotopomer distribution in product metabolites that derive from a given stable isotope-labeled precursor molecule and the degree of enrichment at each site with good precision. Together with modeling tools, this information provides a rich functional biochemical readout of cellular activity and how it responds to external influences. In this chapter, we describe NMR- and MS-based methodologies for isotopomer analysis in metabolomics and its applications for different biological systems.",

author = "Lane, {Andrew N.} and Fan, {Teresa W.M.} and Higashi, {Richard M.}",

note = "Funding Information: This work was supported in part by grants from the Susan G. Komen Foundation Grant BCTR0503648, Kentucky Science and Engineering Foundation, KSEF‐296‐RDE‐3, NSF EPSCoR EPS‐0447479, NCI 1RO1 CA101199, and the Brown Foundation.",

year = "2008",

doi = "10.1016/S0091-679X(07)84018-0",

language = "English",

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series = "Methods in Cell Biology",

pages = "541--588",

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Isotopomer-Based Metabolomic Analysis by NMR and Mass Spectrometry. / Lane, Andrew N.; Fan, Teresa W.M.; Higashi, Richard M.
Biophysical Tools for Biologists, Volume One: In Vitro Techniques. ed. / John Correi; William Detrich, III. 2008. p. 541-588 (Methods in Cell Biology; Vol. 84).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

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Isotopomer-Based Metabolomic Analysis by NMR and Mass Spectrometry

Abstract

Publication series

Bibliographical note

Funding

ASJC Scopus subject areas

Access to Document

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