Nuclear magnetic resonance (NMR) and mass spectrometry (MS) together are synergistic in their ability to profile comprehensively the metabolome of cells and tissues. In addition to identification and quantification of metabolites, changes in metabolic pathways and fluxes in response to external perturbations can be reliably determined by using stable isotope tracer methodologies. NMR and MS together are able to define both positional isotopomer distribution in product metabolites that derive from a given stable isotope-labeled precursor molecule and the degree of enrichment at each site with good precision. Together with modeling tools, this information provides a rich functional biochemical readout of cellular activity and how it responds to external influences. In this chapter, we describe NMR- and MS-based methodologies for isotopomer analysis in metabolomics and its applications for different biological systems.
|Title of host publication||Biophysical Tools for Biologists, Volume One|
|Subtitle of host publication||In Vitro Techniques|
|Editors||John Correi, William Detrich, III|
|Number of pages||48|
|State||Published - 2008|
|Name||Methods in Cell Biology|
Bibliographical noteFunding Information:
This work was supported in part by grants from the Susan G. Komen Foundation Grant BCTR0503648, Kentucky Science and Engineering Foundation, KSEF‐296‐RDE‐3, NSF EPSCoR EPS‐0447479, NCI 1RO1 CA101199, and the Brown Foundation.
ASJC Scopus subject areas
- Cell Biology