Issues concerning the large scale cryopreservation of peripheral blood mononuclear cells (PBMC) for immunotherapy trials

Angela Best, Giovi Hidalgo, Katherine Mitchell, John R. Yannelli

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Immunotherapy of cancer is being developed as an alternative or adjuvant to conventional therapies such as: surgery, chemotherapy and/or radiation treatment. Immunotherapy laboratories routinely process and prepare for injection large numbers of anti-tumor effector cells. The process of cryopreservation is critical to the success of immunotherapy. Standardized safe procedures are required. In the current report, we show the ability to cryopreserve peripheral blood mononuclear cells (PBMC) in Plasmalyte-A, a fluid replacement medium approved by the FDA. These studies show that this medium can be used in place of human serum in terms of cell recovery, cell surface phenotype and response to PHA. However, T cell cytokine release stimulated through the CD3 receptor was altered following the cryopreservation process. These results are important toward the improvement of cryopreservation techniques for their use in immunotherapy.

Original languageEnglish
Pages (from-to)294-297
Number of pages4
JournalCryobiology
Volume54
Issue number3
DOIs
StatePublished - Jun 2007

Bibliographical note

Funding Information:
This work was supported by Kentucky Lung Cancer Research Fund (KLCR) and funds from the Markey Cancer Center, University of Kentucky.

Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.

Funding

This work was supported by Kentucky Lung Cancer Research Fund (KLCR) and funds from the Markey Cancer Center, University of Kentucky.

FundersFunder number
Kentucky Lung Cancer Research Fund
University of Kentucky Markey Cancer Center
University of Kentucky

    Keywords

    • Cryopreservation
    • Dendritic cells (DCs)
    • Immunotherapy
    • Plasmalyte-A
    • Vaccines

    ASJC Scopus subject areas

    • General Biochemistry, Genetics and Molecular Biology
    • General Agricultural and Biological Sciences

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