TY - JOUR
T1 - Juvenile hormone membrane signaling phosphorylates USP and thus potentiates 20-hydroxyecdysone action in Drosophila
AU - Gao, Yue
AU - Liu, Suning
AU - Jia, Qiangqiang
AU - Wu, Lixian
AU - Yuan, Dongwei
AU - Li, Emma Y.
AU - Feng, Qili
AU - Wang, Guirong
AU - Palli, Subba R.
AU - Wang, Jian
AU - Li, Sheng
N1 - Publisher Copyright:
© 2021 Science China Press
PY - 2022/1/30
Y1 - 2022/1/30
N2 - Juvenile hormone (JH) and 20-hydroxyecdysone (20E) coordinately regulate development and metamorphosis in insects. Two JH intracellular receptors, methoprene-tolerant (Met) and germ-cell expressed (Gce), have been identified in the fruit fly Drosophila melanogaster. To investigate JH membrane signaling pathway without the interference from JH intracellular signaling, we characterized phosphoproteome profiles of the Met gce double mutant in the absence or presence of JH in both chronic and acute phases. Functioning through a potential receptor tyrosine kinase and phospholipase C pathway, JH membrane signaling activated protein kinase C (PKC) which phosphorylated ultraspiracle (USP) at Ser35, the PKC phosphorylation site required for the maximal action of 20E through its nuclear receptor complex EcR-USP. The uspS35A mutant, in which Ser was replaced with Ala at position 35 by genome editing, showed decreased expression of Halloween genes that are responsible for ecdysone biosynthesis and thus attenuated 20E signaling that delayed developmental timing. The uspS35A mutant also showed lower Yorkie activity that reduced body size. Altogether, JH membrane signaling phosphorylates USP at Ser35 and thus potentiates 20E action that regulates the normal fly development. This study helps better understand the complex JH signaling network.
AB - Juvenile hormone (JH) and 20-hydroxyecdysone (20E) coordinately regulate development and metamorphosis in insects. Two JH intracellular receptors, methoprene-tolerant (Met) and germ-cell expressed (Gce), have been identified in the fruit fly Drosophila melanogaster. To investigate JH membrane signaling pathway without the interference from JH intracellular signaling, we characterized phosphoproteome profiles of the Met gce double mutant in the absence or presence of JH in both chronic and acute phases. Functioning through a potential receptor tyrosine kinase and phospholipase C pathway, JH membrane signaling activated protein kinase C (PKC) which phosphorylated ultraspiracle (USP) at Ser35, the PKC phosphorylation site required for the maximal action of 20E through its nuclear receptor complex EcR-USP. The uspS35A mutant, in which Ser was replaced with Ala at position 35 by genome editing, showed decreased expression of Halloween genes that are responsible for ecdysone biosynthesis and thus attenuated 20E signaling that delayed developmental timing. The uspS35A mutant also showed lower Yorkie activity that reduced body size. Altogether, JH membrane signaling phosphorylates USP at Ser35 and thus potentiates 20E action that regulates the normal fly development. This study helps better understand the complex JH signaling network.
KW - 20-Hydroxyecdysone action
KW - Juvenile hormone
KW - Phosphoproteomics
KW - Receptor tyrosine kinase
KW - USP
KW - Yorkie activity
UR - http://www.scopus.com/inward/record.url?scp=85110433409&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85110433409&partnerID=8YFLogxK
U2 - 10.1016/j.scib.2021.06.019
DO - 10.1016/j.scib.2021.06.019
M3 - Article
AN - SCOPUS:85110433409
SN - 2095-9273
VL - 67
SP - 186
EP - 197
JO - Science Bulletin
JF - Science Bulletin
IS - 2
ER -