Kinetic modeling of selenium reduction by a defined co-culture in batch reactors

Yuxia Ji, Yi Tin Wang

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

The kinetics of Se(VI) reduction by a defined co-culture of a Se(VI)-reducing strain Shigella fergusonii TB42616 (TB) and a Se(IV)-reducing strain Pantoea vagans EWB32213-2 (EWB) was investigated in batch reactors. Se(VI) reduction was a two-stage process from Se(VI) to Se(IV) and then from Se(IV) to Se(0). Biokinetic parameters were optimized with a Monod-type kinetic model using batch pure culture data through the Robust Global Optimization Algorithm embedded in a computer package including yield coefficients, decay coefficients, maximum specific growth rates, and half-velocity constants. Se(VI) reduction by the defined co-culture was then simulated and verified over a range of culture compositions and initial Se(VI) concentrations, respectively. An inter-species inhibition term was incorporated into the model to illustrate the competition for Se(IV) between these two species during Se(VI) reduction in the co-culture. The results indicated that the incubation time corresponding to the peak level of Se(IV) increased with initial Se(VI) concentrations and a significant increase of Se(IV) accumulation with higher initial Se(VI) concentration. However, Se(IV) accumulation can be reduced with increasing population ratio of EWB to TB. The relatively high correlation coefficients suggested that the model was robust and applicable in simulating Se(VI) reduction by the defined co-culture.

Original languageEnglish
Pages (from-to)17-24
Number of pages8
JournalPROCESS BIOCHEMISTRY
Volume87
DOIs
StatePublished - Dec 2019

Bibliographical note

Funding Information:
This research was supported by a U.S. Geological Survey Grant from the Kentucky Water Resources Research Institute awarded to Yi-tin Wang and a teaching assistantship from the Department of Civil Engineering at the University of Kentucky. We are also grateful to Dr. David Price for providing wastewater samples from the Town Branch Wastewater Treatment Plant.

Funding Information:
This research was supported by a U.S. Geological Survey Grant from the Kentucky Water Resources Research Institute awarded to Yi-tin Wang and a teaching assistantship from the Department of Civil Engineering at the University of Kentucky. We are also grateful to Dr. David Price for providing wastewater samples from the Town Branch Wastewater Treatment Plant.

Publisher Copyright:
© 2019 Elsevier Ltd

Keywords

  • Defined co-culture
  • Kinetic modeling
  • Pantoea vagans
  • Selenium reduction
  • Shigella fergusonii

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

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