Knockout BR-C induces premature expression of E93 thus triggering adult differentiation under larval morphology

Chang Liu; Mian Zhi Wu; Zi Jing Zheng; Shu Ting Fan; Jin Fang Tan; Yaoyu Jiao; Subba Reddy Palli; Guan Heng Zhu

doi:10.1002/ps.8592

Knockout BR-C induces premature expression of E93 thus triggering adult differentiation under larval morphology

Chang Liu, Mian Zhi Wu, Zi Jing Zheng, Shu Ting Fan, Jin Fang Tan, Yaoyu Jiao, Subba Reddy Palli, Guan Heng Zhu

Research output: Contribution to journal › Article › peer-review

Abstract

BACKGROUND: Holometabolan pupal-specifier broad-complex (BR-C) and adult specifier ecdysone-induced protein 93F (E93) are essential for metamorphosis; however, their interaction and effects on programmed cell death and cell differentiation during pupation remain unclear. RESULTS: Here, multiple single-guide RNA (sgRNA)-mediated mosaic knockout of BR-C induced a deformed larva/pupa intermediate phenotype in Spodoptera frugiperda. Quantitative real-time polymerase chain reaction (qPCR) analysis showed that the adult specifier E93 was prematurely expressed in the BR-C mutants during the penultimate and last instar larval stages. Additionally, histological observation and TUNEL assay showed that apoptosis in the fat body and midgut was activated in the larval tissues; astonishingly, the adult midgut appeared in the pupae of BR-C mutants. CONCLUSION: Overall, the results demonstrated that the premature expression of E93 induced by lack of BR-C triggers adult differentiation during the larval stages, which revealed the inhibitory effect of BR-C on E93 during metamorphosis in S. frugiperda.

Original language	English
Journal	Pest Management Science
DOIs	https://doi.org/10.1002/ps.8592
State	Accepted/In press - 2024

Bibliographical note

Publisher Copyright:
© 2024 Society of Chemical Industry.

Keywords

CRISPR/Cas9
Spodoptera frugiperda
apoptosis
metamorphosis
sgRNA

ASJC Scopus subject areas

Agronomy and Crop Science
Insect Science

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10.1002/ps.8592

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@article{483ab79da24e419ab30f77db239be17d,

title = "Knockout BR-C induces premature expression of E93 thus triggering adult differentiation under larval morphology",

abstract = "BACKGROUND: Holometabolan pupal-specifier broad-complex (BR-C) and adult specifier ecdysone-induced protein 93F (E93) are essential for metamorphosis; however, their interaction and effects on programmed cell death and cell differentiation during pupation remain unclear. RESULTS: Here, multiple single-guide RNA (sgRNA)-mediated mosaic knockout of BR-C induced a deformed larva/pupa intermediate phenotype in Spodoptera frugiperda. Quantitative real-time polymerase chain reaction (qPCR) analysis showed that the adult specifier E93 was prematurely expressed in the BR-C mutants during the penultimate and last instar larval stages. Additionally, histological observation and TUNEL assay showed that apoptosis in the fat body and midgut was activated in the larval tissues; astonishingly, the adult midgut appeared in the pupae of BR-C mutants. CONCLUSION: Overall, the results demonstrated that the premature expression of E93 induced by lack of BR-C triggers adult differentiation during the larval stages, which revealed the inhibitory effect of BR-C on E93 during metamorphosis in S. frugiperda.",

keywords = "CRISPR/Cas9, Spodoptera frugiperda, apoptosis, metamorphosis, sgRNA",

author = "Chang Liu and Wu, {Mian Zhi} and Zheng, {Zi Jing} and Fan, {Shu Ting} and Tan, {Jin Fang} and Yaoyu Jiao and Palli, {Subba Reddy} and Zhu, {Guan Heng}",

note = "Publisher Copyright: {\textcopyright} 2024 Society of Chemical Industry.",

year = "2024",

doi = "10.1002/ps.8592",

language = "English",

}

TY - JOUR

T1 - Knockout BR-C induces premature expression of E93 thus triggering adult differentiation under larval morphology

AU - Liu, Chang

AU - Wu, Mian Zhi

AU - Zheng, Zi Jing

AU - Fan, Shu Ting

AU - Tan, Jin Fang

AU - Jiao, Yaoyu

AU - Palli, Subba Reddy

AU - Zhu, Guan Heng

PY - 2024

Y1 - 2024

N2 - BACKGROUND: Holometabolan pupal-specifier broad-complex (BR-C) and adult specifier ecdysone-induced protein 93F (E93) are essential for metamorphosis; however, their interaction and effects on programmed cell death and cell differentiation during pupation remain unclear. RESULTS: Here, multiple single-guide RNA (sgRNA)-mediated mosaic knockout of BR-C induced a deformed larva/pupa intermediate phenotype in Spodoptera frugiperda. Quantitative real-time polymerase chain reaction (qPCR) analysis showed that the adult specifier E93 was prematurely expressed in the BR-C mutants during the penultimate and last instar larval stages. Additionally, histological observation and TUNEL assay showed that apoptosis in the fat body and midgut was activated in the larval tissues; astonishingly, the adult midgut appeared in the pupae of BR-C mutants. CONCLUSION: Overall, the results demonstrated that the premature expression of E93 induced by lack of BR-C triggers adult differentiation during the larval stages, which revealed the inhibitory effect of BR-C on E93 during metamorphosis in S. frugiperda.

AB - BACKGROUND: Holometabolan pupal-specifier broad-complex (BR-C) and adult specifier ecdysone-induced protein 93F (E93) are essential for metamorphosis; however, their interaction and effects on programmed cell death and cell differentiation during pupation remain unclear. RESULTS: Here, multiple single-guide RNA (sgRNA)-mediated mosaic knockout of BR-C induced a deformed larva/pupa intermediate phenotype in Spodoptera frugiperda. Quantitative real-time polymerase chain reaction (qPCR) analysis showed that the adult specifier E93 was prematurely expressed in the BR-C mutants during the penultimate and last instar larval stages. Additionally, histological observation and TUNEL assay showed that apoptosis in the fat body and midgut was activated in the larval tissues; astonishingly, the adult midgut appeared in the pupae of BR-C mutants. CONCLUSION: Overall, the results demonstrated that the premature expression of E93 induced by lack of BR-C triggers adult differentiation during the larval stages, which revealed the inhibitory effect of BR-C on E93 during metamorphosis in S. frugiperda.

KW - CRISPR/Cas9

KW - Spodoptera frugiperda

KW - apoptosis

KW - metamorphosis

KW - sgRNA

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U2 - 10.1002/ps.8592

DO - 10.1002/ps.8592

M3 - Article

AN - SCOPUS:85210960791

SN - 1526-498X

JO - Pest Management Science

JF - Pest Management Science

ER -

Knockout BR-C induces premature expression of E93 thus triggering adult differentiation under larval morphology

Abstract

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Keywords

ASJC Scopus subject areas

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