TY - JOUR
T1 - Ku complex interacts with and stimulates the Werner protein
AU - Cooper, Marcus P.
AU - Machwe, Amrita
AU - Orren, David K.
AU - Brosh, Robert M.
AU - Ramsden, Dale
AU - Bohr, Vilhelm A.
N1 - Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 2000/4/15
Y1 - 2000/4/15
N2 - Werner syndrome (WS) is the hallmark premature aging disorder in which affected humans appear older than their chronological age. The protein WRNp, defective in WS, has helicase function, DNA-dependent ATPase, and exonuclease activity. Although WRNp functions in nucleic acid metabolism, there is little or no information about the pathways or protein interactions in which it participates. Here we identify Ku70 and Ku86 as proteins that interact with WRNp. Although Ku proteins had no effect on ATPase or helicase activity, they strongly stimulated specific exonuclease activity. These results suggest that WRNp and the Ku complex participate in a common DNA metabolic pathway.
AB - Werner syndrome (WS) is the hallmark premature aging disorder in which affected humans appear older than their chronological age. The protein WRNp, defective in WS, has helicase function, DNA-dependent ATPase, and exonuclease activity. Although WRNp functions in nucleic acid metabolism, there is little or no information about the pathways or protein interactions in which it participates. Here we identify Ku70 and Ku86 as proteins that interact with WRNp. Although Ku proteins had no effect on ATPase or helicase activity, they strongly stimulated specific exonuclease activity. These results suggest that WRNp and the Ku complex participate in a common DNA metabolic pathway.
KW - Exonuclease activity
KW - Ku proteins
KW - WRNp
KW - Werner syndrome
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M3 - Article
C2 - 10783163
AN - SCOPUS:0034655912
SN - 0890-9369
VL - 14
SP - 907
EP - 912
JO - Genes and Development
JF - Genes and Development
IS - 8
ER -