Abstract
This study evaluated the in vitro effect of L-canavanine on cell cycle progression in the two human pancreatic cancer cells lines PANC-1 and MIA PaCa-2. After 72 h of exposure to L-canavanine, the percentage of cells in the radiosensitive G2/M phase of the cell cycle increased 6-fold in PANC-1 cells and 4-fold in MIA PaCa-2 cells, when compared to untreated cells. The capacity of L-canavanine to redistribute cells into the G2/M phase of the cell cycle was both concentration- and time-dependent. Since many drugs that cause cells to accumulate in the G2/M phase of the cell cycle are effective radiosensitization agents, the potential of L-canavanine to synergistically enhance the effects of ionizing radiation also was evaluated. The interaction between these treatment modalities was quantified using the median-effect equation and combination index analysis. L-Canavanine was found to be synergistic with radiation when either PANC-1 or MIA PaCa-2 cells were exposed to L-canavanine for 72 h prior to irradiation. These results suggest that L-canavanine in combination with radiation may have clinical potential in the treatment of pancreatic cancer.
Original language | English |
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Pages (from-to) | 37-43 |
Number of pages | 7 |
Journal | Molecular and Cellular Biochemistry |
Volume | 244 |
Issue number | 1-2 |
DOIs | |
State | Published - Feb 2003 |
Bibliographical note
Funding Information:The authors gratefully acknowledge the American Foundation for Pharmaceutical Education Fellowships for fellowship support of AKB.
Keywords
- Cell cycle analysis
- Ionizing radiation
- L-canavanine
- Median-effect analysis
- Pancreatic cancer
- Radiosensitization
ASJC Scopus subject areas
- Molecular Biology
- Clinical Biochemistry
- Cell Biology