Labeling of major plant lipids and jasmonic acid using [1- 14C] lauric acid

Meshack M. Afitlhile, Hirotada Fukushige, David Hildebrand

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


A medium chain length fatty acid, [1- 14C] lauric acid (12:0) was administered to the detached leaves of Artemisia and was incorporated into major lipids, including phospholipids and galactolipids. [1- 14C]12:0 was elongated and desaturated into linolenic acid (18:3). In detached leaves of both Artemisia and Arabidopsis thaliana ecotype Columbia, radioactivity from [ 14C]18:3 was incorporated into jasmonic acid (JA) and methyl jasmonate (MJ). Higher amounts of [ 14C]JA were measured in Artemisia than Arabidopsis leaves. In Artemisia, [ 14C]JA was actively metabolized into [ 14C]MJ. Extracts prepared from the leaves of Artemisia, exhibited higher in vitro JA methyltransferase activity than those from Arabidopsis.

Original languageEnglish
Pages (from-to)2679-2684
Number of pages6
Issue number19
StatePublished - Oct 2004


  • Arabidopsis thaliana: Cruciferae
  • Artemisia tridentata: Compositae
  • JA methyltransferase
  • Jasmonic acid
  • Lauric acid

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Plant Science
  • Horticulture


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