TY - JOUR
T1 - Laboratory and clinical evaluation of assays for β-hydroxybutyrate
AU - Porter, William H.
AU - Yao, H. Harry
AU - Karounos, Dennis G.
PY - 1997/3
Y1 - 1997/3
N2 - We have performed a two-part evaluation of assays for β-hydroxy- butyrate (βHB). In Part I, we evaluated the analytical acceptability of a dry film, self-contained analyzer for βHB measurement (KetoSite; GDS Diagnostics, Elkhart, Ind). The assay response is linear and between-day imprecision is acceptable. Acetoacetate (AcAc) interferes severely with this assay. This interference could be minimized by dilution of serum before the assay. With this predilution protocol, KetoSite βHB results compare favorably with an automated Sigma (St. Louis, Mo) βHB assay. In Part II, we compared the clinical utility of βHB measurements (Sigma) with the qualitative measurement of ketones using the nitroprusside test (Acetest; Bayer, Elkhart, Ind) for the diagnosis and management of diabetic ketoacidosis (DKA). Test results for both assays were abnormal for all patients with DKA on admission. However, frequent monitoring with either test during the management of uncomplicated DKA added little if any additional clinical information over routine measurements of serum glucose and total carbon dioxide.
AB - We have performed a two-part evaluation of assays for β-hydroxy- butyrate (βHB). In Part I, we evaluated the analytical acceptability of a dry film, self-contained analyzer for βHB measurement (KetoSite; GDS Diagnostics, Elkhart, Ind). The assay response is linear and between-day imprecision is acceptable. Acetoacetate (AcAc) interferes severely with this assay. This interference could be minimized by dilution of serum before the assay. With this predilution protocol, KetoSite βHB results compare favorably with an automated Sigma (St. Louis, Mo) βHB assay. In Part II, we compared the clinical utility of βHB measurements (Sigma) with the qualitative measurement of ketones using the nitroprusside test (Acetest; Bayer, Elkhart, Ind) for the diagnosis and management of diabetic ketoacidosis (DKA). Test results for both assays were abnormal for all patients with DKA on admission. However, frequent monitoring with either test during the management of uncomplicated DKA added little if any additional clinical information over routine measurements of serum glucose and total carbon dioxide.
KW - Acetoacetate
KW - Assay evaluation
KW - Diabetic ketoacidosis
KW - Interference
KW - β- hydroxybutyrate
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U2 - 10.1093/ajcp/107.3.353
DO - 10.1093/ajcp/107.3.353
M3 - Article
C2 - 9052387
AN - SCOPUS:0031057860
SN - 0002-9173
VL - 107
SP - 353
EP - 358
JO - American Journal of Clinical Pathology
JF - American Journal of Clinical Pathology
IS - 3
ER -