Laboratory Maintenance and Propagation of Freshwater Planarians

Makayla R.P. Dean, Elizabeth M. Duncan

Research output: Contribution to journalArticlepeer-review


Freshwater planarians are a powerful model organism for the study of animal regeneration, stem cell maintenance and differentiation, and the development and functions of several highly conserved complex tissues. At the same time, planarians are easy to maintain, inexpensive to propagate, and reasonably macroscopic (1 mm to 1 cm in length), making them excellent organisms to use in both complex academic research and hands-on teaching laboratories. Here, we provide a detailed description of how to maintain and propagate these incredibly versatile animals in any basic laboratory setting.

Original languageEnglish
Article numbere120
JournalCurrent Protocols in Microbiology
Issue number1
StatePublished - Dec 2020

Bibliographical note

Publisher Copyright:
© 2020 Wiley Periodicals LLC


  • Girardia
  • Schmidtea mediterranea
  • planarian
  • regeneration

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Virology


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