Lack of Offspring Nrf2 Does Not Exacerbate the Detrimental Metabolic Outcomes Caused by In Utero PCB126 Exposure

Brittany B. Rice, Sara Y. Ngo Tenlep, Obadah Tolaymat, Attaas T. Alvi, Fallon R. Slone, Claire L. Crosby, Stevi S. Howard, Cecile L. Hermanns, Nishimwe P. Montessorie, Hollie I. Swanson, Kevin J. Pearson

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Human environmental exposures to toxicants, such as polychlorinated biphenyls (PCBs), increase oxidative stress and disease susceptibility. Such exposures during pregnancy and/or nursing have been demonstrated to adversely affect offspring health outcomes. Nuclear factor erythroid-2-related factor 2 (Nrf2) regulates the antioxidant response and is involved in the detoxification of coplanar PCBs, like PCB126. The purpose of this study was to investigate glucose tolerance and body composition in PCB-exposed offspring expressing or lacking Nrf2. We hypothesized that offspring lacking Nrf2 expression would be more susceptible to the long-term health detriments associated with perinatal PCB exposure. During gestation, whole-body Nrf2 heterozygous (Het) and whole-body Nrf2 knockout (KO) mice were exposed to vehicle or PCB126. Shortly after birth, litters were cross-fostered to unexposed dams to prevent PCB exposure during nursing. Offspring were weaned, and their body weight, body composition, and glucose tolerance were recorded. At two months of age, PCB exposure resulted in a significant reduction in the average body weight of offspring born to Nrf2 Het dams (p < 0.001) that primarily arose from the decrease in average lean body mass in offspring (p < 0.001). There were no differences in average body weight of PCB-exposed offspring born to Nrf2 KO dams (p > 0.05), and this was because offspring of Nrf2 KO dams exposed to PCB126 during pregnancy experienced a significant elevation in fat mass (p = 0.002) that offset the significant reduction in average lean mass (p < 0.001). Regardless, the lack of Nrf2 expression in the offspring themselves did not enhance the differences observed. After an oral glucose challenge, PCB-exposed offspring exhibited significant impairments in glucose disposal and uptake (p < 0.05). Offspring born to Nrf2 Het dams exhibited these impairments at 30 min and 120 min, while offspring born to Nrf2 KO dams exhibited these impairments at zero, 15, 30, 60 and 120 min after the glucose challenge. Again, the interactions between offspring genotype and PCB exposure were not significant. These findings were largely consistent as the offspring reached four months of age and demonstrate that the lack of offspring Nrf2 expression does not worsen the metabolic derangements caused by in utero PCB exposure as we expected. Future directions will focus on understanding how the observed maternal Nrf2 genotypic differences can influence offspring metabolic responses to in utero PCB exposure.

Original languageEnglish
Article number777831
JournalFrontiers in Endocrinology
Volume12
DOIs
StatePublished - Dec 16 2021

Bibliographical note

Funding Information:
This study and core services were supported by US NIH grants (National Institute of General Medical Sciences, P20GM103527, and the National Institute of Environmental Health Sciences, P42ES007380 and P30ES026529). The Southern Regional Education Board Doctoral Scholars Program supported BR. CH was supported by the SURES (Summer Undergraduate Research in Environmental Health Sciences) program funded by NIEHS R25ES027684. NM was supported by Berea College Office of Internships & Career Development.

Funding Information:
We thank Brittnany Graham, Marissa McDowell, Tyler Gripshover, Yu Li, and Dr. Andrew Morris for their assistance in generating the preliminary data that led to the successful design and implementation of this project. We also thank Dr. Brett Spear for his useful discussions and comments on the manuscript. We also thank Dr. Viviana Perez for providing the Nrf2 Het breeding pairs, and Dr. Masayuki Yamamoto for producing the original lines and giving us permission to use them. Parts of this manuscript will be published online by the University of Kentucky as a section of BR?s PhD dissertation in order to fulfill the requirements of her dissertation.

Publisher Copyright:
Copyright © 2021 Rice, Ngo Tenlep, Tolaymat, Alvi, Slone, Crosby, Howard, Hermanns, Montessorie, Swanson and Pearson.

Keywords

  • DOHAD
  • developmental programming
  • diabetes
  • mice
  • nuclear factor erythroid-2-related factor 2 (Nrf2)
  • obesity
  • polychlorinated biphenyals (PCBs)
  • pregnancy

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism

Fingerprint

Dive into the research topics of 'Lack of Offspring Nrf2 Does Not Exacerbate the Detrimental Metabolic Outcomes Caused by In Utero PCB126 Exposure'. Together they form a unique fingerprint.

Cite this